FastQCFastQC Report
Fri 20 Dec 2024
1D8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10088635
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC2874122.84886904918257Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC2825872.801042955761607Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA934390.9261807965101324Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC899740.8918352185404667Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA635590.6300059423301567No Hit
CACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAG605280.5999622347324489No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTGGGGGG520360.5157883103115536TruSeq Adapter, Index 12 (97% over 45bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT491580.4872611607021167No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT486430.4821564066893093No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC459830.4557901044095658Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGA445850.4419329274971292No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA430250.42646998330299396No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAG405150.4015905025803788No Hit
ACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAAC381920.37856459273231713Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC351490.3484019394100391No Hit
TTCTTTATGCTGTAGGTAAAAGCCCAAAAAAAAGATCGGAAGAGCACACG338240.3352683489887383No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT337850.33488177538388497No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC322470.3196368983514618No Hit
CACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTCTGA287510.2849840439266561Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAAACTTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG277270.27483400876332625TruSeq Adapter, Index 12 (100% over 37bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG268980.2666168416242633Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT268540.26618070730083904Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTAGGGGG265370.263038557743441TruSeq Adapter, Index 12 (97% over 45bp)
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA234620.23255871582230897No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT219370.21744269665817031Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCATGGAACTTCGAAGTACATATTCAGCAGATCGGAAGAGCACACGTC218250.21633253656218113No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTGGGGGGGGGGG209910.20806580870454724TruSeq Adapter, Index 12 (97% over 41bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC201350.1995810136852012No Hit
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA200210.19845102930178363No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC198940.19719218705008162No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG193650.19194866302527547Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAAGAGCACA192390.19069973291728762No Hit
ACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTCTGAA188900.18724039476103554Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC185590.1839594751916389No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGCAGATCGGAAGAGCACACGT184450.18282949080822133No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC176290.17474118153744286No Hit
GTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGG174880.1733435692737422No Hit
AAAGGATTCTAATAATGGTAGTTTTGATAAGATCGGAAGAGCACACGTCT172820.1713016676686192Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGGATTCTAATAATGGTAGTTTTGAGATCGGAAGAGCACACGTCTGAA170120.1686253888657881Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTTTATGTGCGGAACCTGTCAACCAAGTAGATCGGAAGAGCACACGTC155100.15373734900707578No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT150540.14921741147340548No Hit
CCACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGA147140.1458472826105811Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACCCCTCCCCGCACATTGACCTCTAAGATCGGAAGAGCACACGTCTGAA146780.14549044543687029Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA141540.14029648213063511No Hit
TTACATCTACCTTTGAAAAATTCGAAAAGTAGATCGGAAGAGCACACGTC141170.1399297328132101No Hit
CACCCCTCCCCGCACATTGACCTCTAGATCGGAAGAGCACACGTCTGAAC140970.13973148993892634Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGGATTCTAATAATGGTAGTAGATCGGAAGAGCACACGTCTGAACTCC137170.1359648753275344Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACCCCTCCCCGAACATTGACCTCATAAGATCGGAAGAGCACACGTCTGA135930.13473576950697494Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGTCAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT132480.13131607992557962Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCCCTCCCCGAACATTGACCTCATAGATCGGAAGAGCACACGTCTGAA130010.12886778042817487Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG127820.12669702095476743No Hit
TGAAGACGAAGCTCAACCTGAGGAAAATCAGATCGGAAGAGCACACGTCT120220.11916379173198358Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTCTTCTGAGGCGGAAGATCGGAAGAGCACACGTCTGAACT118990.11794459805513828Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG117130.11610093932429906TruSeq Adapter, Index 12 (100% over 37bp)
AAAGGATTCTAATAATGGTAGTTTTAGATCGGAAGAGCACACGTCTGAAC116690.11566480500087475Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACCTCGGATCAGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC114880.11387070698860649Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACCTCGGATCAGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT114500.11349404552746728Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTAATTGTTTACTCGCGATGGAAAAAAAAAAGATCGGAAGAGCACACGTC110510.10953910018550578No Hit
TTCTTTATGCTGTAGGTAAAAGCCCAAAAAAAAAGATCGGAAGAGCACAC109460.10849832509551588No Hit
CACCCCTCCCCGCACATTGACCTCATAAGATCGGAAGAGCACACGTCTGA108420.10746746214924022Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTTTATGAGTGGAACCTCTCGACCAAGAGATCGGAAGAGCACACGTCT108300.10734851642466994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTGCTCTGAGGTGGAAGATCGGAAGAGCACACGTCTGAACT106980.10604011345439696Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACCCCTCCCCGCACATTGACCTCATAGATCGGAAGAGCACACGTCTGAA106780.10584187058011317Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTATGGAATTTAAGGGACATACGACGAGTAGATCGGAAGAGCACACGTCT106750.1058121341489706Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCCCTCCCCGCACATTGACCTCTTTAGATCGGAAGAGCACACGTCTGA104500.103581901813278Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTTTATGTGCAGAACCTGTCGACCAAGTAGATCGGAAGAGCACACGTC104110.10319532820842463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)