FastQCFastQC Report
Fri 20 Dec 2024
1D6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17740927
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA4062162.2897112422592123Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3053021.7208909094772782No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1034930.5833573409100888Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT1022860.5765538632789595No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG1020790.5753870696835628No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT952320.5367926940909007Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC949800.535372249713896No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA940800.5302992340817365Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT902460.5086881874887372Illumina RNA PCR Primer (100% over 50bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT899110.5067998983367668No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC865670.48795082692127645Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG803010.452631364753375Illumina DpnII expression Sequencing Primer (100% over 23bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA765670.431583986563949Illumina RNA PCR Primer (100% over 45bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT745750.4203557119647694Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG710060.40023838664123923No Hit
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC569830.3211951664081589Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT540560.30469659223556916Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC520610.2934514075842824No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA349550.19703029046903806No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT339610.1914274265375197No Hit
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA335340.18902056245426185Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCTGAATATGTACTTCGAAGTTCCATGAGAGATCGTCGGACTGTAGAACT308370.17381842560989064No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA300860.1695852758990553Illumina DpnII expression Sequencing Primer (100% over 24bp)
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA292730.1650026517780046No Hit
TTTCATTTGTATCCTCACGGTTGACCCCTGATCGTCGGACTGTAGAACTC289830.16336801340764212Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT288430.16257887764263954No Hit
GATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGA275640.15536955876093736Illumina DpnII expression Sequencing Primer (100% over 24bp)
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC275450.15526246176425842No Hit
ACTGTTCTCGCTGTTATTATATTCAACCAAGATCGTCGGACTGTAGAACT258630.14578155921615596No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT256970.14484586966622431Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG251860.14196552412396488No Hit
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT240390.13550024753497944No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT238210.1342714504151897No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT235400.13268754220114878No Hit
TCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC230400.1298692001832824Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGGATCGTCGGACT221770.12500474186044505No Hit
GCAAATTCTGTTGCAAATCTCACGTTCTAAGATCGTCGGACTGTAGAACT218860.12336446680604683No Hit
CTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAACG217670.12269370140579464Illumina Small RNA Adapter 1 (100% over 26bp)
CCTTCTACCATTATTATTCCTAGGTCTCAGATCGTCGGACTGTAGAACTC213640.12042211773939435Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTCGAATGCTCTACCACTGAGCTACACCCCCGATCGTCGGACTGTAGAA211840.11940751461296244No Hit
TTCCGCCTCAGAAGAAAGATTTCCAGATCGTCGGACTGTAGAACTCTGAA205720.115957863983094Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC204060.11502217443316237Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTCATTTGTATCCTCACGGTTAACCCCTGATCGTCGGACTGTAGAACTC201660.11366937026458651Illumina DpnII expression Sequencing Primer (100% over 21bp)
TAAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTG198820.1120685519984384Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC194940.10988151859257411No Hit
AGGCTGATCCGAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG188590.10630222422988382Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAACT188090.10602039002809717No Hit
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC187600.10574419251034627No Hit
GGCTGATCCGAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA184010.10372062294151821Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACTTGGAATAAAGATCAGAATTACACAGATCGTCGGACTGTAGAACTC183550.10346133547587452Illumina DpnII expression Sequencing Primer (100% over 21bp)
CATCAAGTTTTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG183260.10329787163883826Illumina RNA PCR Primer (100% over 33bp)
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG182360.10279057007562233No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG179700.1012912121221174No Hit
CCCTTCTACCATTATTATTCCTAGGTCTCAGATCGTCGGACTGTAGAACT178430.10057535324957934No Hit
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG178000.10033297583604284Illumina RNA PCR Primer (100% over 33bp)
GCTACTTCAGATGTAGTATCGCCAAATCGTAGATCGTCGGACTGTAGAAC177500.10005114163425619No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)