FastQCFastQC Report
Fri 20 Dec 2024
1D6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17740927
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC3843412.1664087789775586Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3010201.6967546284362707No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA1015680.5725067241413033No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC983480.5543566015462439No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC978180.5513691590073055Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT918580.5177745221543384No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC889440.5013492248742132Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC886220.49953421261470726Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC875550.49351987074858034No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT827790.46659906779392085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA761130.42902493201172637Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG714580.4027861678253904Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC693840.3910956851352807No Hit
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT539300.30398637004706686Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG506240.2853514926249344Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT506160.2853063991526486No Hit
TATGATTGAAATCTTCATGAACGACACAAAAGGCCAAAAAAAAAAAAAAA367340.20705795136860664No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG339970.19163034716280608No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC325980.18374462619681597No Hit
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC316840.17859269698815627Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCATGGAACTTCGAAGTACATATTCAGCAGATCGGAAGAGCACACGTC295760.16671056704083165No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA291230.1641571491726447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG286150.16129371368249246No Hit
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA282300.15912359032873535No Hit
AGGGGTCAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT277560.15645180209579804Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG271370.15296269467767945No Hit
GAGGTCCGGACTTGGGGAGGGTTATCAGATCGGAAGAGCACACGTCTGAA262100.14773748857655522Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA261020.14712872670069607No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA250150.1410016511538546No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTAGGGGG249290.14051689632678158TruSeq Adapter, Index 9 (97% over 36bp)
TTGGTTGAATATAATAACAGCGAGAACAGTAGATCGGAAGAGCACACGTC247860.1397108505096718No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG246480.13893298811274066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC229460.12933935188392354No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC222870.12562477710437567No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAG217560.12263169788140157No Hit
TGAAAATCTTTCTTCTGAGGCGGAAGATCGGAAGAGCACACGTCTGAACT216230.12188201890464911Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAGAACGTGAGATTTGCAACAGAATTTGCAGATCGGAAGAGCACACGTC211330.11912004372714008No Hit
GGGGGTGTAGCTCAGTGGTAGAGCATTCGACTAGATCGGAAGAGCACACG205160.11564220967709296No Hit
TTAACGAGTAGATAAATGAAGAGAGATCGGAAGAGCACACGTCTGAACTC204330.11517436490212717Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGACCTAGGAATAATAATGGTAGAAGGAGATCGGAAGAGCACACGTCT200770.1131677053854063Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT195470.11018026284646794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC193360.10899092251492834Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGGTTAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT193170.10888382551824942Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTCTGA190070.10713645346717227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT188560.10628531417777663No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT185240.10441393507791336No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAGATCGGAAGAGCACACGTC179410.10112774828508116No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA178800.10078391055890147No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTAGTGGG177970.10031606578393565TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)