FastQCFastQC Report
Fri 20 Dec 2024
1D4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19267536
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA7440513.8616821580092027No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG3729071.9354161320887113No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3381191.7548637251800125No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT2356491.2230365107401382Illumina RNA PCR Primer (100% over 50bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1812490.9406963090661931Illumina DpnII expression Sequencing Primer (100% over 25bp)
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG1517820.7877603031337271No Hit
AAAGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAACTCT1487360.7719513278708808Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1394920.7239742538952568No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1227490.6370767907219688Illumina RNA PCR Primer (100% over 45bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT1199230.6224096324511863Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA1177180.6109655121443655No Hit
GGTAAAGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAAC1169860.6071663756071352No Hit
GGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA982770.5100652205865867No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC964690.5006815609427173No Hit
CAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGA854950.4437256533476828Illumina DpnII expression Sequencing Primer (100% over 24bp)
AAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGT845590.4388677410541753No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC782590.40617025446325883Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG765680.3973938338560779No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT591290.3068840769260792Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA562020.2916927208543947No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC557100.2891392028539612No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC553420.2872292544308727No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG498160.25854888762112604No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT462210.23989055995535702Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAACTCTGA428980.22264393329795776Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA412070.21386751269077686No Hit
AAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGAA403050.2091860630233155Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA399390.20728649475470035Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT398900.2070321809701043No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC364460.18915755496706999No Hit
GAGCGCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAG358640.1861369300153377No Hit
GGCGAGAATTCTACCATTGAACCACCAGTGCGATCGTCGGACTGTAGAAC358080.18584628569008513No Hit
CATCAAGTTTTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG338800.17583981677781735Illumina RNA PCR Primer (100% over 33bp)
ACAACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGT336720.17476028071259345No Hit
AGAACCGGTGTTTTCCCTGTGGTATGGTCGTAGACGATCGTCGGACTGTA333040.172850332289505No Hit
AAGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAACTCTG322360.16730732980075916Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG322020.16713086717471295No Hit
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC314740.16335249094642926Illumina RNA PCR Primer (100% over 32bp)
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC302100.15679223331929937No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC297830.15457607033924836No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG292380.15174747824527227No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT269140.13968573874728973No Hit
AGCGCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGA266140.13812871557629372No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG261110.13551810672625705Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACAACGGTTCAGAGTTGAATTCAGACAGGAGATCGTCGGACTGTAGAAC252590.13109616092062834No Hit
GTATCAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACT245040.12717765260695504No Hit
TATCAGACTAACATGAGATATCAGCATCAAAGCTAAGACAGGGGTAACTG239160.12412588719180283No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA228780.11873858702015659No Hit
AACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAG218000.11314368375904423No Hit
AGTGATATGCTTAAGTTCGGCGGGTGATCCAGGCTGATCCGAGGTCCGGA217760.11301912190536455No Hit
GACGGATCCGTTCAGTTACTCGGAGTCGCCCGACTCGGTTCTGATCGTCG212210.1101386290390219No Hit
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT211050.10953658007957011No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGGAT210200.10909542351445456No Hit
TATCAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTC207500.10769410266055815Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC203930.10584124508707289No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG203820.10578415423746969No Hit
GCAAAGGTGTTTATGCTGCAGCCTAGATCGTCGGACTGTAGAACTCTGAA201790.10473056855842906Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAGGCGGGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTA200660.10414408983068722No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGATCGTCGGAC198740.10314759500124977No Hit
ACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGAACGT195850.10164766267985693Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT193040.10018925097635732No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)