FastQCFastQC Report
Fri 20 Dec 2024
1D4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19267536
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC7368653.824386262986611No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA3703731.9222644763710315No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3344501.7358213317987314No Hit
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC1712710.8889097183988652Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA1541050.7998168525544729No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTAGATCGGAAGAGCACACGTCTG1433540.7440183321832122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1363230.7075268991323022No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC1161940.6030558344357058No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACCAGATCGGAAGAGCACACGT1146760.5951772971904659No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC1093810.5676958382223861Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCCAGATCGGAAGAGCAC996130.5169991637747556No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT956200.4962751853687986No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAGATCGGAAGAGCA839700.4358107855617864No Hit
AAAGGATTCTAATAATGGTAGTTTTGAGATCGGAAGAGCACACGTCTGAA804350.41746386253021667Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC753040.39083357622894804No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT747750.38808802537075837Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG555760.28844373250424965Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC552750.28688151925601696No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG550290.2856047602558002No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA548640.2847483975117524No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA494150.256467666649228No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG442210.22951040548205023Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGATGAAGAAATCTTATGTAAGCTAGATCGGAAGAGCACACGTCTGAA412970.21433461964207567Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAAGATCGGAAGAGCAC409410.21248695214582702No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC392200.20355482922154652No Hit
AAAGGATTCTAATAATGGTAGTTTTAGATCGGAAGAGCACACGTCTGAAC386070.200373311875478Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC379540.19698419143994333Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTGGGGTG369190.19161246150000705TruSeq Adapter, Index 7 (97% over 37bp)
TCCCATATGGTCTAGTGGCTAGGATCCGGCGCTCAGATCGGAAGAGCACA360650.18718013553990503No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT357840.18572172383640545No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT353420.18342770969780464No Hit
GTCTACGACCATACCACAGGGAAAACACCGGTTCTAGATCGGAAGAGCAC331270.17193168861861735No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTGTAGATCGGAAGAGCA329010.17075873116313367No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTAGATCGGAAGAGCACA318500.16530395998741093No Hit
TAAAACTTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAA312350.1621120624868691TruSeq Adapter, Index 7 (97% over 37bp)
ATGGATGAAGAAATCTTATGTAAGCTTAGATCGGAAGAGCACACGTCTGA309610.16068998132402607Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTGGGGGG301240.15634588667694718TruSeq Adapter, Index 7 (97% over 37bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT297930.15462797111161491No Hit
GCCCGGCTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAA296840.15406225269281967TruSeq Adapter, Index 2 (97% over 36bp)
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA291120.15109352851345392No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT290230.15063161163939176No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCGCTAGATCGGAAGAGCACAC263140.1365716924052977No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC256510.1331306711973965No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA249170.1293211545056929Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTGTCTGAATTCAACTCTGAACCGTTGTCAGATCGGAAGAGCACACGT248540.1289941796397837No Hit
AGTGGTGATGATATCCCAGTTACCCCTGTCTTAGCTTTGATGCTGATATC247060.12822604820875902No Hit
AAAGGATTCTAATAATGGTAGTTTTGATACAGATCGGAAGAGCACACGTC235200.12207061660608808No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCCGAACTTAAGCATATCACTAG227040.1178355135809789No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG224300.11641343241813587No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTAGATCGGAAGAGCACA213030.11056421537242749No Hit
AGAACCGAGTCGGGCGACTCCGAGTAACTGAACGGATCCGTCAGATCGGA212630.11035661228296136No Hit
CCTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGA210000.10899162196972151No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC202080.104881080798292No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT199780.10368736303386172No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGA197380.1024417444970649No Hit
AAAGGATTCTAATAATGGTAGTTTTGATAAGATCGGAAGAGCACACGTCT196660.10206805893602587Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGCTGCAGCATAAACACCTTTGCAGATCGGAAGAGCACACGTCTGAAC194970.10119093588303142Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)