FastQCFastQC Report
Fri 20 Dec 2024
1D3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21747242
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA3382761.5554891972048686Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT2640271.2140711911882895Illumina RNA PCR Primer (100% over 50bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2447331.1253518951966415No Hit
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA1777360.8172806464378334No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1702330.7827797198375775Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG1499970.6897288400984364Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA1261040.5798620349191865No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT1199660.551637766296986Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1176660.5410617125610686No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG1166900.5365737871496533No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG1073710.49372237638225575No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC1055320.4852661316777548Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG1045520.4807598131294074No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1000920.46025146545019363Illumina RNA PCR Primer (100% over 45bp)
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG986630.4536805172812258No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT865710.3980780643356983No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC810220.37256218512673933No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA803240.3693525827321Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT757230.34819587697603216Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT738900.3397672219769293No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC597450.2747244915010372No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC535330.2461599498455942No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG533430.24528627584132276No Hit
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC515700.23713351789620035Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT476550.21913123512397573Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC451210.20747918287753456No Hit
AGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC441540.2030326420242162Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC434460.19977705678724686No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT428550.19705947080553937No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT416200.19138058977777503No Hit
CATCAAGTTTTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG399760.18382101049871058Illumina RNA PCR Primer (100% over 33bp)
ACACGCTTTGTCCATAACAAGAGACCATAGATCGTCGGACTGTAGAACTC372760.17140564306959016Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC361600.1662739578655537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG334360.1537482316148411No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA328480.15104444048583265No Hit
GAAGAACGAAAGTTTCAATCATCATACCAGGATCGTCGGACTGTAGAACT325820.14982129687985263No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT310350.14270775117138992No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA305100.14029365194906093Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGGAT285770.13140516852665732No Hit
TTCCGCCTCAGAAGAAAGATTTCCAGATCGTCGGACTGTAGAACTCTGAA282960.13011305065718218Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC272320.12522047623326213Illumina RNA PCR Primer (100% over 32bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA267040.12279258215823413No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT256440.11791840087124611Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT255750.1176011192591686Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG255380.1174309827425473No Hit
GCAAAGGTGTTTATGCTGCAGCCTAGATCGTCGGACTGTAGAACTCTGAA248060.11406503868398576Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT247590.11384891932503441No Hit
AATGAACCAATCAGACAATTGTTGAGAAGATCGTCGGACTGTAGAACTCT244740.11253840831862727Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT242980.11172911029361791Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCCAGTTCAATTGTAAAAGACTACAGTCAGATCGTCGGACTGTAGAACT242760.11162794804049177No Hit
CTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACTC241530.11106235907983183Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA241340.11097499167940468No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACGATCGTC237950.10941617332441511No Hit
TATCATTTGTGTTCTTGACAAGTGGAATCAGATCGTCGGACTGTAGAACT235400.10824361084499819No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT222130.10214168766779713Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACTC220190.10124962052659367Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)