FastQCFastQC Report
Fri 20 Dec 2024
1D3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21747242
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC3140471.4440773685233281Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2334461.0734510610586852No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC1707120.7849822979851881No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC1578000.7256092519685945Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1406330.6466705065405535Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGGGGG1245410.5726749166629957TruSeq Adapter, Index 20 (97% over 39bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC1209890.5563418110673528No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1146800.5273312358413081No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA1138190.5233721131166885No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC1096810.5043444129605031Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA1024050.4708872968811401No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC1017760.46799497609857843No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA998500.4591386806658058No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT989890.4551795579411863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC820320.37720645220207694No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA782560.3598433309382404No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC742240.34130304891075386Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGG737530.33913725703700726TruSeq Adapter, Index 20 (97% over 43bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG705820.32455609773414024Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC702590.32307085192687884No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG584800.26890766194628263No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA526230.2419755111935573No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT501820.23075109938078586No Hit
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT492670.22654366930758393Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG444370.20433395646215738Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT428020.19681576174118998No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT427810.19671919777229682No Hit
TAGTAACAATGACCTGATCTGGAGACTCTAGATCGGAAGAGCACACGTCT425540.1956753872514041Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC404380.1859454178143601No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC392800.18062060467253732No Hit
TAAAACTTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGG359230.16518416450233092TruSeq Adapter, Index 20 (100% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT353020.1623286299936332No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGTAGATCGGAAGAGCACACGTCT350420.16113307609305125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC313010.14393089477736995No Hit
CTGGTATGATGATTGAAACTTTCGTTCTTCAGATCGGAAGAGCACACGTC309530.1423306918642833No Hit
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA295540.13589769222230572No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA291640.13410436137143275Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGA284810.1309637332402886No Hit
TGGAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC261780.12037388465167215Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG255130.11731602563672212No Hit
GCCCGGCTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG253370.1165067276117128TruSeq Adapter, Index 19 (100% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG239610.11017948850709437Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCCAGATCGGAAGAGCAC239170.10997716400084204No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG239130.10995877086391001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC234310.10774239786360035No Hit
TCCAAGACCTGAGTTACCACTGACAAGTAGATCGGAAGAGCACACGTCTG233600.10741591968305682Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGG233480.10736074027226072No Hit
TAGGCTGCAGCATAAACACCTTTGCAGATCGGAAGAGCACACGTCTGAAC232940.10711243292367831Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTCAACAATTGTCTGATTGGTTCATTAGATCGGAAGAGCACACGTCTG229840.10568696481144596Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTGTAGTCTTTTACAATTGAACTGGGCAGATCGGAAGAGCACACGTC227580.10464775257478627No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGAGATCGGAAGAGCACACGTCT222370.10225204648938932Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATTCCACTTGTCAAGAACACAAATGATAAGATCGGAAGAGCACACGTC220510.10139676562204991No Hit

[FAIL]Adapter Content

Adapter graph