FastQCFastQC Report
Fri 20 Dec 2024
1D10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10538690
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2270682.154613144517962Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC956770.9078642601689583No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT766960.7277564858630436Illumina RNA PCR Primer (100% over 50bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG694410.6589149125745231No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT545530.5176449824408915Illumina DpnII expression Sequencing Primer (100% over 22bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA505090.47927209169261076Illumina RNA PCR Primer (100% over 45bp)
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT403710.3830741771510501No Hit
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG380250.360813345871261Illumina RNA PCR Primer (100% over 33bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG372480.35344051300493706Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT346240.32854178270733836Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA332400.3154092206906171Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC323830.3072772801932688No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC307210.29150681915873794Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG294590.27953189627932884No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT291380.2764859769098436No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG275240.2611709804539274No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC275070.2610096700823347No Hit
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT266660.25302955111119124No Hit
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC259250.2459983166788282Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT236950.22483819146402448Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCCAGTTCAATTGTAAAAGACTACAGTCAGATCGTCGGACTGTAGAACT196980.18691127644897043No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA183890.1744903778363345Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCTATTTTCAGTAAGGACCAACTAAGCCAGATCGTCGGACTGTAGAACTC180520.17129263694064442Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAGACCCAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG170800.16206947922369858No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG167910.1593272029066231Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC163770.15539882091607213Illumina RNA PCR Primer (100% over 32bp)
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA163390.15503824479133554No Hit
GCTTCCTATGAGGTCACATGAGAACCAATCAGATCGTCGGACTGTAGAAC138830.13173364051888803No Hit
GCTCCAGATTGAGACAACAGATACGTAAGATCGTCGGACTGTAGAACTCT136900.12990229335904177Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC136480.129503761852754No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC133180.12637244287477856No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA130150.12349732272227383No Hit
TGTCGAAGTTGGATATGTCCATGGTCTCAGATCGTCGGACTGTAGAACTC122370.11611500101056202Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT119520.11341068007503778No Hit
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA112990.10721446403680154No Hit
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT111710.10599989182716257No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG107520.10202406560967255No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTAGATCGTCGGACTG106780.10132189105097503No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)