FastQCFastQC Report
Fri 20 Dec 2024
1D10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10538690
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC2130322.02142771065474Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT932860.8851764308467183No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC680490.6457064397946993No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG516830.4904119961778931Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA396920.3766312511327309No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA353550.3354781286858234Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAA351970.3339788911145503TruSeq Adapter, Index 7 (97% over 37bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC311300.2953877569223499Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC310300.29443887238356947Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT308150.2923987706251916No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT292610.2776531048925436Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA291150.2762677334659241No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC280950.2665891111703637No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA273270.2593016779125299No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT270830.25698639963790565No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC256120.24302830807244546No Hit
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT244890.23237233470194113Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG223620.21218956056208127Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTGTAGTCTTTTACAATTGAACTGGGCAGATCGGAAGAGCACACGTC188300.17867495865235622No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA177550.16847444986046653Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGCTTAGTTGGTCCTTACTGAAAATAGCAGATCGGAAGAGCACACGTCT169080.16043739781699623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATACGATCCACTGAGATTCATCCTGTGTTTTTGGGTCTGTAGATCGGAA168930.16029506513617917No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA159770.15160328276095036Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG157060.1490318056608554No Hit
GCCCGGCTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAA151230.14349980879976543TruSeq Adapter, Index 2 (97% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT131660.12493013837583228No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGCAGATCGGAAGAGCACACGT131200.12449365148799328No Hit
TTACGTATCTGTTGTCTCAATCTGGAGCAGATCGGAAGAGCACACGTCTG129920.12327907927835434Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT128320.12176086401630562No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTGGGGTG127950.12140977673695687TruSeq Adapter, Index 7 (97% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG126610.12013827145499108No Hit
TGAGACCATGGACATATCCAACTTCGACAAGATCGGAAGAGCACACGTCT115540.10963411961069165Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC114380.10853341354570634No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC111190.10550647186699676No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA106380.10094233723546284No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC105990.10057227226533848No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)