FastQCFastQC Report
Fri 20 Dec 2024
1C4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20385525
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2245681.1016051830894718Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2172491.0657022568709906No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT991090.4861733999982831Illumina RNA PCR Primer (100% over 50bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA677120.3321572537376398Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACCGCTACTCTACCAATTGAGCTACACCCGTGATCGTCGGACTGTAGAA668500.3279287631787752No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA665970.3266876864834239Illumina RNA PCR Primer (100% over 45bp)
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG581020.28501596108022725No Hit
AATCTAGCGCGTCTACCAATTCCGCCACATCCGCGATCGTCGGACTGTAG529790.2598853843597356No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT495750.24318726154955536No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC473010.23203228761584505No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG460270.22578275516573648No Hit
AGCCACGTGCTCTAGTCCACTGAGCTACACGCCCGATCGTCGGACTGTAG445070.21832648411066186No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG421530.2067790748582634Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC375780.18433668007078552No Hit
CAAAGTAGTGCGCTACCAAGCTGCGCTACGCCCCGGATCGTCGGACTGTA339240.16641219688970482No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT321920.15791597224010664Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCACGTGCTCTAGTCCACTGAGCTACACGCCCGATCGTCGGACTGTAGA310590.1523581070391859No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT302970.14862016062868139No Hit
GCTCCCCAGGCAGGATTCGAACCTGCGACCAATCGGTTAGATCGTCGGAC291900.14318983690633427No Hit
TCCACCGTGAGCCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC278550.136641072525726Illumina RNA PCR Primer (100% over 30bp)
GCTCCCCGAAGCGTATCGTCGGTAACCACGTCCTTGATCGTCGGACTGTA274710.1347573829960229No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC252660.12394088452468112No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT248670.12198361337272402No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA238330.11691138687867984No Hit
AGTCCTCTGCTCTACCGACTGAGCTATCCCGGCGATCGTCGGACTGTAGA216250.10608017208288724No Hit
CAGGGCGGTGCTCTAACCGATTGAACTACAGGCCCGATCGTCGGACTGTA210240.1031320017512426No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)