FastQCFastQC Report
Fri 20 Dec 2024
1C4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20385525
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2159001.0590848163096118No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC2152891.0560875915631311Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC646120.3169503851384745Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA593660.2912164391154999No Hit
ACGGGTGTAGCTCAATTGGTAGAGTAGCGGTCAGATCGGAAGAGCACACG568520.2788841592257251No Hit
GCGGATGTGGCGGAATTGGTAGACGCGCTAGATTAGATCGGAAGAGCACA522280.25620139780555073No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC488220.23949346411240327No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC449980.2207350558791103No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT435040.21340632630260933No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA403760.19806210534190316Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT368410.18072136969737104No Hit
CGGGGCGTAGCGCAGCTTGGTAGCGCACTACTTTGAGATCGGAAGAGCAC331070.16240445119760222No Hit
GGGCGTGTAGCTCAGTGGACTAGAGCACGTGGCTAGATCGGAAGAGCACA328140.1609671568429069No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG303760.14900769050588591Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA297420.14589764060528243No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTATCTGGGGGG296220.14530898762725022TruSeq Adapter, Index 27 (97% over 43bp)
GGGCGTGTAGCTCAGTGGACTAGAGCACGTGGCAGATCGGAAGAGCACAC293060.14375886811843208No Hit
TAACCGATTGGTCGCAGGTTCGAATCCTGCCTGGGGAGCAGATCGGAAGA280330.13751424110980706No Hit
AAGGACGTGGTTACCGACGATACGCTTCGGGGAGCAGATCGGAAGAGCAC265250.13011683535253568No Hit
AGGGCTCACGGTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA265110.13004815917176527Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT247540.12142929848507704No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC233000.11429678656792013No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC231460.11354134857944546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)