FastQCFastQC Report
Fri 20 Dec 2024
1C10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11581215
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA3430642.9622453257279133No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG2274391.9638613047076667No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1885591.6281452334664368No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG792630.6844100554216461Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT761500.6575303195735508No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC629850.5438548546072238No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT622580.5375774476166792Illumina RNA PCR Primer (100% over 50bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA620230.5355482995523354No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC609610.5263782772360239No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG568260.49067390597618643Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA558220.48200469467150037No Hit
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT532590.459874028761231No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT507570.4382700778804296Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCGCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGA465500.40194401019236753No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA453350.3914528829660791No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA420750.36330385024369205Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA411130.35499729518880363No Hit
AAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGT406080.3506367855186179No Hit
CGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA392200.3386518599300678No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG355480.3069453420906183No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA321030.27719889493459887Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA313280.2705070236585712Illumina RNA PCR Primer (100% over 45bp)
GAGCGCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAG312300.26966082574237676No Hit
AACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAG290310.2506731806636868No Hit
CGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACTCT285740.24672713527898413Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGT283240.24456846712542685No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT272690.23545888751741506No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC256880.22180747011431873No Hit
AGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTGA250200.21603950880801367Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT247490.21369951252955757No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC246640.21296556535734806No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC217340.18766597459765666No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGGAT214010.1847906286171183No Hit
GCTACGTCTGCAGGCTACAACCCAGGACCAGGGCGATCGTCGGACTGTAG209760.18112089275607093No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG207640.17929034216185435Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG189620.1637306621110134No Hit
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC175920.15190116062951944No Hit
TGCTCAGGAAGAAGTACAGAGTACCGATGATCGTCGGACTGTAGAACTCT168340.14535607878793372Illumina DpnII expression Sequencing Primer (100% over 22bp)
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC166870.14408678191364205No Hit
GGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA158560.1369113689712176Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC155510.13427779382387772No Hit
TGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA151930.1311865810279837No Hit
CGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA141140.12186976927723041No Hit
AGAGCGCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTA138750.11980608252242964No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT132210.11415900663272376Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCTCAGATATAGATCATTAACAGCCTAGGCCAATCGGGGGATCGTCGGA125460.1083306026181191No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA123170.1063532625894606No Hit
AGTTCGGCGGGTGATCCAGGCTGATCCGAGGTCCGGATCGTCGGACTGTA121280.1047213094653713No Hit
GGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTG120830.10433274919773097Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAACTC118860.10263171869272784Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTAG118400.1022345237524733Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGGCGGGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTA117220.10121563238399424No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)