FastQCFastQC Report
Fri 20 Dec 2024
1C10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11581215
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC3347902.8908020445177813No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA2244491.9380436335911218No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1867471.6124992066894537No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA759530.6558292890685476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC749560.6472205204721612No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA617280.5330010711311378No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG607670.5247031507488635No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT569540.4917791440708078No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA547690.4729123844087171Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC546650.47201437845683725No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC525990.45417514483583976No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG489300.42249453101423295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCATATGGTCTAGTGGCTAGGATCCGGCGCTAGATCGGAAGAGCACAC420190.3628203085772952No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC415220.3585288762880233No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCCAGATCGGAAGAGCAC413280.3568537498008629No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAGATCGGAAGAGCA397730.3434268338857365No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT397370.34311598567162427Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCGAGATCGGAAGAGCAC374390.3232735080041257No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC348660.301056495367714No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCGCTCAGATCGGAAGAGCACA311440.26891824389755303No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC306200.264393675447697Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTAGATCGGAAGAGCACA282920.2442921576017715No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTGTAGATCGGAAGAGCA277250.23939629822950356No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGAGATCGGAAGAGCACACGTCTG271190.23416368662528067Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC259310.22390569555957646No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT251540.2171965549383204No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG242250.20917494407970147No Hit
GCACTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA241130.20820786074690784Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC231730.2000912684895324No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT215590.18615490689016653No Hit
CCTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGA213270.18415166284366538No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA200540.17315972460575166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCTGGTCCTGGGTTGTAGCCTGCAGACGTAGCAGATCGGAAGAGCACA200380.17302156984392397No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTAGATCGGAAGAGCACA189700.16379973949192725No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT168560.1455460415854468No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT164280.1418504017065567No Hit
ATCGGTACTCTGTACTTCTTCCTGAGCAAGATCGGAAGAGCACACGTCTG163060.14079697164762073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTCGCGCTAGTCCCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT152930.13205004828940659Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT152370.13156650662300975No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAAGATCGGAAGAGCAC151160.13052171123668804No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCGCTCTAGATCGGAAGAGCAC137120.11839863088631028No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG136690.11802733996389844No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC125310.10820108252890565Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCGATTGGCCTAGGCTGTTAATGATCTATATCTGAGGTAGATCGGAAG123520.10665547613095863No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC120770.1042809411620456No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCAGATCGGAAGAGCACACGTCTGA116360.10047305053917054Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)