FastQCFastQC Report
Fri 20 Dec 2024
1B9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12196471
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC6681205.477978015116012No Hit
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA6233425.1108390287649605No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3549392.910177870303631No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG2761692.264335314698817No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG2489822.0414265733096073Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1776321.4564212877643048No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1148810.9419200029254363No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1123030.9207827411716061No Hit
AACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAG1029610.8441868143662212No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT956140.7839480780957049No Hit
GGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA937100.7683370050238303No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA923220.7569566639399217No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT911480.7473309287579989Illumina RNA PCR Primer (100% over 50bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT841670.6900930605254586Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA708840.5811845082073331No Hit
ACAACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGT686060.5625069743534831No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA627570.5145504793968682Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA517150.42401609449159516Illumina RNA PCR Primer (100% over 45bp)
AAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGT473130.387923687105885No Hit
AAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG462840.3794868204089527No Hit
AGCGCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGA449070.36819666934804335No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC421790.34582954364422297No Hit
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG381160.31251662878549047Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA368430.3020791833965743No Hit
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC347660.28504966723571107No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT321990.2640025955048801Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG308720.25312239909396744No Hit
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT308620.25304040816396806No Hit
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT301080.2468582920420177No Hit
AGCCCGACGACTTACCCTTCGTCCTCTCTACGATCGTCGGACTGTAGAAC286230.2346826389371155No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA283340.23231310106013453Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA261550.21444727741327796No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG242780.19905757985240158Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC235410.19301484831145008No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT234800.1925147036384541No Hit
AGAACCGGTGTTTTCCCTGTGGTATGGTCGTAGACGATCGTCGGACTGTA208030.17056573167763034No Hit
GCAGTCGAATGCTCTACCACTGAGCTATACCCCCGATCGTCGGACTGTAG205040.16811420287065004No Hit
ACAGGCGGGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTA189120.15506124681475486No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAGATCGT188430.15449550939775938No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCAGATCGTCGGACTG187720.15391337379476408No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT176820.14497636242483586Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA165170.13542441907991254No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162590.13330905308592952No Hit
TAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG155650.12761888254397524No Hit
AACGCAGGGTCCTAACCACTAGACGATCGCGGCGATCGTCGGACTGTAGA154320.12652840317498398No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA152760.12524934466699425No Hit
AGTTCGGCGGGTGATCCAGGCTGATCCGAGGTCCGGATCGTCGGACTGTA150870.1236997160900067No Hit
GAGCGCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAG148040.12137937277102533No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA140620.11529564576507419No Hit
GACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAG138360.11344265074708906No Hit
TGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG137770.11295890426009293Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGT136750.11212259677409965Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC136390.11182742942610203Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT129960.10655541262714437No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT129090.10584209153615008No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC128470.10533374777015417No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)