FastQCFastQC Report
Fri 20 Dec 2024
1B9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12196471
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT6284205.152474023018626No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC5929754.861857171635959No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT3429062.8115181842354233No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA2612632.1421196344417988No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA2308891.8930803836617986Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1659191.360385311456076No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1108360.9087546717407027No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC1063890.8722933051699956No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTAGATCGGAAGAGCACA973130.797878337102593No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCCAGATCGGAAGAGCAC923440.7571370439859202No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC918100.7527587283239554No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG874700.7171746647042411No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG776020.6362660149808907Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC675940.5542094922375497No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTGTAGATCGGAAGAGCA648850.5319981493007281No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC574130.47073452640522Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAGATCGGAAGAGCA451210.3699512752500293No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTAGATCGGAAGAGCACA441900.3623179196670906No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCGCTAGATCGGAAGAGCACAC429160.35187227518517444No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA407010.3337112841903203No Hit
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA368730.30232515618657235Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAAGATCGGAAGAGCAC357240.292904398329648No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT324760.2662737442658618No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGGGGGGGGGG317210.26008342905091153TruSeq Adapter, Index 18 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGGGGG304520.24967878003399507TruSeq Adapter, Index 18 (97% over 45bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC297300.24375903488804262No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG293190.24038920766506966Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC289920.2377081042540912No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC282700.23178835910813875No Hit
GTAGAGAGGACGAAGGGTAAGTCGTCGGGCTAGATCGGAAGAGCACACGT268420.22008005430423275No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT255340.2093556406603189Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG245190.2010335612653857No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT222900.18275778296853246No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA222060.18206905915653798Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC220410.18071620881154885No Hit
GTCTACGACCATACCACAGGGAAAACACCGGTTCTAGATCGGAAGAGCAC198970.16313735341969No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTCGACTGCAGATCGGAAGAGCACA191400.15693064001873985No Hit
TGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCG186330.15277369986777323No Hit
TGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGC179240.14696054293081992No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCCCGCCTGTAGATCGGAAGAGCAC169640.13908941365088312No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCAGATCGGAAGAGCACAC162060.13287450115693303No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC157340.1290045292609641Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG150500.12339634964900913No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAAGATCGGAAGAGCACA147460.12090382537702914No Hit
GCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTAGATCGGAAGAGCACAC143850.11794395280405291No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCCGAACTAGATCGGAAGAGCAC143140.1173618172010576No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCGCTCAGATCGGAAGAGCACA141990.11641892150606516No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACG137450.11269653328409505No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTCAGATCGGAAGAGCACA134550.11031879631411413No Hit
GGCTGTTTGGTCTAGTGGTATGATTCAAGATCGGAAGAGCACACGTCTGA131990.108219828506131Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT127030.10415307837816365Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTAGATCGGAAGAGCACACGTCTGAACTCC126970.10410388382016404Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG125090.10256245433617642No Hit
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA124290.1019065268961817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)