FastQCFastQC Report
Fri 20 Dec 2024
1B5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16716297
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT1942421.1619917975853145Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1321880.7907732196909399No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1028210.6150943597137571Illumina RNA PCR Primer (100% over 50bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA931540.5572645664287971Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT863660.5166574870020556No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG819530.4902580996257724No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA800480.4788620350547732No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT697160.4170540879956847Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG584580.3497066365834491Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC556090.33266338830902564Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA550510.3293253284504337Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT433350.25923803579225707No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC415220.24839233234489672No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC407140.24355872595467765No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA379500.22702396350100743Illumina RNA PCR Primer (100% over 45bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT364070.2177934503078044Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC352940.21113527714900016Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC346920.2075340011008419No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC342160.20468648050462374No Hit
TCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT322240.19276996574061828Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT284210.17001971190150547No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA266030.15914409752351252No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC257080.1537900409402872No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA250050.14958456409335155Illumina DpnII expression Sequencing Primer (100% over 25bp)
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC237500.14207692050458304No Hit
ACTGTTCTCGCTGTTATTATATTCAACCAAGATCGTCGGACTGTAGAACT215950.12918530940195666No Hit
TGTCGAAGTTGGATATGTCCATGGTCTCAGATCGTCGGACTGTAGAACTC202880.12136659213460971Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG198040.11847121404937949No Hit
TATCAGACTAACATGAGATATCAGCATCAAAGCTAAGACAGGGGTAACTG196480.11753799301364411No Hit
GCTGAATATGTACTTCGAAGTTCCATGAGAGATCGTCGGACTGTAGAACT189370.1132846586776964No Hit
GATCCTGTCTGTCCTGTTCGAGATCGATTAGATCGTCGGACTGTAGAACT182530.1091928433671644No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG177040.10590862318371108No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC174080.1041378960902645No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT168940.10106305242123899No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)