FastQCFastQC Report
Fri 20 Dec 2024
1B5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16716297
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC1798281.0757645667578173Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1297130.7759673090278307No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC874600.523201998624456Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA847670.507091971385768No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC832590.49807083470699276No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC789190.47210814691794484No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG653170.3907384512251726Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA554460.3316882919704047Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT526560.31499799267744527Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC519380.310702783038612Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC419800.2511321735908377No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT409620.2450423081140518No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG401660.24028048795735082No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTGGGGGGGGGGG365460.21862497417938914TruSeq Adapter, Index 12 (96% over 33bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG344660.2061820270362509Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT338450.20246708945168898Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT335390.2006365404969773No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT329020.1968258879343912No Hit
TCCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTCC310040.18547169866627758Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC267520.16003544325636235No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG259990.15553085710310124No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA253970.15192958105494295No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT234080.14003101284931704No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC233880.13991136912678687Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGTTGAATATAATAACAGCGAGAACAGTAGATCGGAAGAGCACACGTC206960.12380732407422529No Hit
AGTGGTGATGATATCCCAGTTACCCCTGTCTTAGCTTTGATGCTGATATC206370.12345437509276126No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC194610.11641932420798698No Hit
TGAGACCATGGACATATCCAACTTCGACAAGATCGGAAGAGCACACGTCT191900.1147981517677031Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTGGGGGG190570.11400252101287744TruSeq Adapter, Index 12 (97% over 37bp)
TCTCATGGAACTTCGAAGTACATATTCAGCAGATCGGAAGAGCACACGTC180260.1078348871164469No Hit
TAATCGATCTCGAACAGGACAGACAGGATCAGATCGGAAGAGCACACGTC173740.1039345017619632No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT171340.10249877709160109No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)