FastQCFastQC Report
Fri 20 Dec 2024
1B2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15441789
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC4606502.9831388060023354No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG3285532.1276874072039194Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG2089711.3532823172237363Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1776801.1506438794106046No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG1494430.9677829427665409No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1384110.8963404434550946Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTGA1312630.850050470188396Illumina DpnII expression Sequencing Primer (100% over 24bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1183270.7662777933308116Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1127890.730414073136215Illumina RNA PCR Primer (100% over 50bp)
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT1011930.6553191472827404No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC949700.6150194125823115No Hit
CGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACTCT833110.539516502912972Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC789050.5109835395367726No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG716890.4642532027862834No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG642450.4160463531783785No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA619290.40104809099515604No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT494600.3202996751218398Illumina DpnII expression Sequencing Primer (100% over 22bp)
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC489990.31731426973908267No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG461360.29877367188477966Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCCATGCACTACCAATGGATAGAATTCTACCAAATTAAAGGTATTGATC436340.28257088605471814No Hit
ACAGACCCAAAAACACAGGATGAATCTCAGTGGATCGTATCACAAAGGGA419390.2715941786278779No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC412730.26728120686016366No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT371400.24051617335271192No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA360000.23313360906563352Illumina RNA PCR Primer (100% over 45bp)
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA353150.22869759455980132Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT350530.22700089996049033Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT306910.19875287766203775No Hit
GGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG298840.19352679925881644Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA298030.19300224863841878No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT296630.19209561793649688Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAAAGCGGAGTTGCGCACATTCATGCTACTAGAGGTCACGATCGTCGGAC285960.1851857968011349No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC283700.18372223581088953No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC268580.17393062423013292No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC255190.16525934915960838No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC251410.16281144626441924No Hit
ACTTATTACTCGAGTTCTTGGAGCCCACTTATTATGGACTTGTGGATCGT246980.1599426076861949No Hit
TGCTCAGGAAGAAGTACAGAGTACCGATGATCGTCGGACTGTAGAACTCT233730.15136199568586256Illumina DpnII expression Sequencing Primer (100% over 22bp)
TAAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACT231470.1498984346956172No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA225540.1460582060796194Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG206350.1336308895297041Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA201560.13052891734241415Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTGAATTTAAGGGTGATAACCCTCCCCAAGTCCGGACCTCGATCGTCG190720.12350900533610451No Hit
TAAGCACTGAAGCAAACATACTCTCAGGAAGTCCCAAGGATCGTCGGACT188970.12237571695870213No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT183980.11914422609970905No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA182190.11798503398796603No Hit
TGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACTCT178660.11569902943240579Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACTC175950.11394405143082839Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT172650.11180699334772674Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC172650.11180699334772674Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACTGAAATTAAGAGTGGATAACCCTCCCCAGGTCCGGACCTCGATCGTC172320.11159328753941658No Hit
ACAGACCCAAAAACACAGGATGAATCTCAGTGGATCGTATCACAAAGGAT169080.10949508505782587No Hit
ACTCGAGTTCTTGGAGCCCACTTATTATGGACTTGTGGATCGTCGGACTG164590.10658739087809062No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG164100.10627007013241795No Hit
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT158540.10266945105907094No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT158120.10239746184849435No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA157200.10180167595865997No Hit
AACTTATTACTCGAGTTCTTGGAGCCCACTTATTATGGACTTGTGGATCG156160.1011281788658037No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA155960.10099866019410057No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT155840.10092094899107869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)