FastQCFastQC Report
Fri 20 Dec 2024
1B2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15441789
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4674573.0272204859164957No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA3254802.1077868632967336Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA2060201.3341718372139395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1791241.1599951275075706No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC1477300.9566896685351678No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC1366480.8849233725444636Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA1296800.8397990673230932Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1184660.7671779480991484Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC1018400.6595090763123366No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT946390.6128758785656248No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGAGATCGGAAGAGCACACGTCTG820090.5310848373850983Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG778780.5043327557448168No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA719120.46569733597577334No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA648500.4199642929973981No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG617860.40012203249247863No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG492330.3188296381980093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT482780.3126451216241849No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA460430.2981714100613601Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATACCTTTAATTTGGTAGAATTCTATCCATTGGTAGTGCATGGCTAGAT431810.27963728814064226No Hit
CCTTTGTGATACGATCCACTGAGATTCATCCTGTGTTTTTGGGTCTGTAG417530.2703896549810388No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT413050.2674884367348887No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC369900.23954478331493842No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC347930.22531715722834966Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG347410.2249804086819215Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGA304840.19741235940991037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC303760.19671295858271343No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC296810.1922121847410297No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC291570.18881879554240769Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGACCTCTAGTAGCATGAATGTGCGCAACTCCGCTTTAAGATCGGAAGA282630.18302931091727778No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT281570.1823428619572512No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT267150.17300456572745554No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT254490.16480603380864742No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT251810.1630704836078255No Hit
CACAAGTCCATAATAAGTGGGCTCCAAGAACTCGAGTAATAAGTAGATCG245200.15878989150803705No Hit
ATCGGTACTCTGTACTTCTTCCTGAGCAAGATCGGAAGAGCACACGTCTG233970.1515174180919063Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACAAGTCCATAATAAGTGGGCTCCAAGAACTCGAGTAATAAGTTGTTTA229060.14833773470159448No Hit
TACCACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTC228920.1482470716314023No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC223010.1444197948825748Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGGGGGGGGG222730.14423846874219043TruSeq Adapter, Index 10 (97% over 41bp)
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA207880.13462170736823303Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT198630.1286314688019633Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGGTCCGGACTTGGGGAGGGTTATCACCCTTAAATTCAGTTAGATCGGA188290.12193535347491148No Hit
CTTGGGACTTCCTGAGAGTATGTTTGCTTCAGTGCTTAAGATCGGAAGAG187260.12126833231564038No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC182510.11819226386269105No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC181460.11751229083624962No Hit
TCCCATATGGTCTAGTGGCTAGGATCCAAGATCGGAAGAGCACACGTCTG179740.11639843025960268Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCT176100.11404119043460573Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT171350.1109651219816564Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG170510.11042114356050327Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGTCCGGACCTGGGGAGGGTTATCCACTCTTAATTTCAGTTAGATCGG169010.10944975352272979No Hit
CACAAGTCCATAATAAGTGGGCTCCAAGAACTCGAGTAGATCGGAAGAGC164260.10637368506978045No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA163700.1060110327890117No Hit
TAAGGATTATTAGTTGGATTTGTGTATTGTGAATGAAAGGTGAAAAGAAT159930.10356960582740769No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC158580.10269535479341158No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC158470.10262411952397484No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC156500.10134836060769901No Hit
ACTACATGGATAACCGTAGTAATTCTAGAGCTAATACATGCAAGGAGTCT156180.10114113073297401No Hit
CACAAGTCCATAATAAGTGGGCTCCAAGAACTCGAGTAATAAGTTAGATC155540.100726670983524No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC154960.10035106683558492No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)