FastQCFastQC Report
Fri 20 Dec 2024
1B1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15391285
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC5997303.896555745670358No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG3020071.9621948394822133Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1955391.2704527269815353No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1447580.9405192613872071No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC1196450.7773554969581812No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1042640.6774223204885103Illumina DpnII expression Sequencing Primer (100% over 25bp)
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC798670.5189105393084463No Hit
ACAGACCCAAAAACACAGGATGAATCTCAGTGGATCGTATCACAAAGGGA575640.3740038599766037No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC572910.37223012893335417No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA546870.3553114635977438No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT544540.35379761988683855Illumina RNA PCR Primer (100% over 50bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT534020.3469625830461849No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT473830.3078560367116846No Hit
GGTCAGAATAAACAATTTTCTCAATCCTACCAAAATCAGATCGTCGGACT458930.29817523358186143No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC458850.2981232561153926No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT418560.27194610456501844Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT397780.2584449576497349Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCCATGCACTACCAATGGATAGAATTCTACCAAATTAAAGGTATTGATC364120.23657543863296665No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA362980.23583475973578552Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA349850.22730395805158568No Hit
GAAGAACGAAAGTTTCAATCATCATACCAGGATCGTCGGACTGTAGAACT344870.22406836076389985No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACTC337080.21900705496649564Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG335880.21822739296946292Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACTGAATTTAAGGGTGATAACCCTCCCCAAGTCCGGACCTCGATCGTCG335280.21783756197094653No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG328370.2133480083046997No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG322400.20946918986946184Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT319270.2074355714938681Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG310320.2016205924326656No Hit
TAAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACT304860.19807313034616666No Hit
AACTGAAATTAAGAGTGGATAACCCTCCCCAGGTCCGGACCTCGATCGTC291790.18958131176181847No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC290360.18865221454868777Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCCGAGATATCCGTCGCTGAAAGTTGTGGATCGTCGGACTGTAGAACTC284160.18462396089735197Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA263350.1711033224321426Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAGACCCAAAAACACAGGATGAATCTCAGTGGATCGTATCACAAAGGAT254080.1650804335050647No Hit
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA249310.1619812770668596No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC247310.16068184040513836No Hit
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG246920.16042845025610272No Hit
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT242470.157537203683773No Hit
AGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAA228330.14835018648540393No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT224580.1459137427446766Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGA221850.14414001170142712Illumina DpnII expression Sequencing Primer (100% over 24bp)
AAAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACT220980.1435747567535784No Hit
GGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTG219390.14254170460751003Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT211290.13727898612753905No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA201560.1309572267682653Illumina RNA PCR Primer (100% over 45bp)
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT190720.12391428006173623No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG172910.11234279658910871No Hit
TAAGCACTGAAGCAAACATACTCTCAGGAAGTCCCAAGGATCGTCGGACT171030.11112132612709075No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA170310.1106535289288711No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGTAATCGATCGTCGGACTGTAGAA159770.10380549772160023No Hit
TGCTCAGGAAGAAATACAGAGTACCGATGATCGTCGGACTGTAGAACTCT158310.10285690895854374Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG156840.10190182301217865No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG154580.10043345958443364No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)