FastQCFastQC Report
Fri 20 Dec 2024
1B1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15391285
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT6038663.923428095834753No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA2966321.9272724791984557Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1956121.2709270213630637No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1445030.9388624796435127No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG1185030.769935713619753No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC1025000.6659612891321289Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT786630.5110879306048846No Hit
CCTTTGTGATACGATCCACTGAGATTCATCCTGTGTTTTTGGGTCTGTAG572340.3718597894847636No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT572330.37185329230145503No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC542860.3527060930909927No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC529880.3442727491564219No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC470920.30596535636888017No Hit
TGATTTTGGTAGGATTGAGAAAATTGTTTATTCTGACCAGATCGGAAGAG455780.29612862083965047No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT455040.29564782927481364No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG409860.26629355508653113Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG394850.2565412829403133Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATACCTTTAATTTGGTAGAATTCTATCCATTGGTAGTGCATGGCTAGAT360420.23417148080878236No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC357050.2319819300337821Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC346530.22514689319312847No Hit
CTGGTATGATGATTGAAACTTTCGTTCTTCAGATCGGAAGAGCACACGTC343980.2234901114494339No Hit
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA336660.2187341732675342Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCT336350.21853276058496743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCACCCTTAAATTCAGTTAGATCGGA330870.21497230413185126No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA317690.20640901653110835Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG316020.20532398691857112Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC306120.19889177544305103No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA304820.19804714161293224No Hit
TACCACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTC300110.19498696827457876No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT286830.18635870884074981Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACCTGGGGAGGGTTATCCACTCTTAATTTCAGTTAGATCGG285530.185514075010631No Hit
CACAACTTTCAGCGACGGATATCTCGGCTAGATCGGAAGAGCACACGTCT283640.18428610736530446Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC256990.1669711138478691Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC249080.16183184185076166No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAGATCGGAAGAGCACA244520.15886912626203725No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT243880.15845330653028647No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC239680.1557244895406719No Hit
CTTTGTGATACGATCCACTGAGATTCATCCTGTGTTTTTGGGTCTGTAGA239510.15561403742442556No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTAGATCGGAAGAGCACACG226330.14705074982368269No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC220270.14311345673866735Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCACCCCTCCCCGCACATTGACCTCTTTAGATCGGAAGAGCACACGTC218930.14224283417531416No Hit
GAGGTCCGGACTTGGGGAGGGTTATCAGATCGGAAGAGCACACGTCTGAA218460.14193746655980966Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGTCCGGACTTGGGGAGGGTTATCCAGATCGGAAGAGCACACGTCTGA216660.14076797356426057Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC210030.13646034103065469No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC189510.12312812088139488No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA174610.11344731775157174No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG169720.11027019511366334No Hit
CTTGGGACTTCCTGAGAGTATGTTTGCTTCAGTGCTTAAGATCGGAAGAG168540.10950352748324782No Hit
GATTACCACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACG157780.10251255824318761No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA156570.10172639906284628No Hit
ATCGGTACTCTGTATTTCTTCCTGAGCAAGATCGGAAGAGCACACGTCTG156050.10138854553079876Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA155090.10076481593317256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)