FastQCFastQC Report
Wed 18 Dec 2024
1B12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10477798
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA6364516.074282019943503Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3843033.667784013396708No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC2675642.553628157366653No Hit
TTCCACTTCAGAGTAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2499422.38544396446658Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2061481.9674744636229864Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1637771.5630860606398407Illumina RNA PCR Primer (100% over 50bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1514261.4452082393647978Illumina RNA PCR Primer (100% over 45bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1260841.2033444431740334Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1190981.1366701285899956No Hit
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1148611.0962322426906874Illumina RNA PCR Primer (100% over 37bp)
GAGGTCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1074591.0255876282402085Illumina RNA PCR Primer (100% over 37bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC684890.6536583354632338No Hit
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT657620.6276318745599028Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGTCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG572560.5464506950792524Illumina RNA PCR Primer (100% over 36bp)
ACTCATCCTAAGTAGACTTTTCACATTGAAGATCGTCGGACTGTAGAACT571730.5456585439039768No Hit
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG551770.5266087397371089Illumina DpnII expression Sequencing Primer (100% over 23bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG527950.5038749554057065No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT519650.49595344365295074No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA489610.4672832975020133No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC477570.45579233346548576No Hit
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG451330.43074890353870154Illumina RNA PCR Primer (100% over 36bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG392300.3744107301935006Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCACTTCAGAGCAAAGATTTCCAGATCGTCGGACTGTAGAACTCTGAA360040.34362181824845256Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT356240.3399951020242994Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATGAGGTCAAAGTAGCGGATGAGGGGTGGATCGTCGGACTGTAGAACTC344110.32841824207719983Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC266970.25479590272688973No Hit
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT238560.22768142695631277Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC238140.2272805793736432Illumina RNA PCR Primer (100% over 32bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA223900.21368993752313223Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC222690.21253511472544137No Hit
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC199740.1906316575295687Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG192060.1833018731607538Illumina Small RNA Adapter 1 (100% over 26bp)
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT188580.17998056461863454Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT188270.17968470092666416Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG182900.17455957826253188No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG171180.1633740219080383Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG169940.16219056714015676Illumina Small RNA Adapter 1 (100% over 26bp)
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGG151470.14456281749275945No Hit
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG148250.1414896526922928Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC143650.1370994172630547Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC140640.13422667625392282No Hit
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA137260.13100080761243918Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCGACAGTCGCCGCAGTGAAAACACGCACAGATCGTCGGACTGTAGAACT125910.12016837889029736No Hit
CCACTCGGCCACGACTACGATCGTCGGACTGTAGAACTCTGAACGTGTAG121510.11596902326233051Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC117850.11247592289906716Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAACTC117190.11184601955487213Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT112200.10708356851315516No Hit
ACCGTCAAAAGTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC111760.10666363295035847Illumina RNA PCR Primer (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110680.10563288202349386No Hit
GATAACCTGAGATTATCAGTCCTCTTTAGATCGTCGGACTGTAGAACTCT110510.10547063419241332Illumina DpnII expression Sequencing Primer (100% over 22bp)
AATCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG107980.10305600470633237Illumina RNA PCR Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)