FastQCFastQC Report
Wed 18 Dec 2024
1B12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10477798
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC6027265.752410955049907Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3816033.642015240225093No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG2650362.5295009504859705No Hit
TGAAAATCTTTACTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC2361712.25401367730128Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC1946381.8576231379913983Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTGGGGGG1327111.2665924653252525TruSeq Adapter, Index 3 (97% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1213861.1585067778554234Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1174631.1210657048360735No Hit
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGAT1079851.0306077670136415TruSeq Adapter, Index 3 (97% over 36bp)
CAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGAT1028760.9818475217789082TruSeq Adapter, Index 3 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTGGGGGGGGGGG855240.8162402061959966TruSeq Adapter, Index 3 (96% over 32bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT673070.6423773392081046No Hit
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC619230.5909924967058918Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAATGTGAAAAGTCTACTTAGGATGAGTAGATCGGAAGAGCACACGTC550670.5255589008301172No Hit
CCAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGA546010.5211114014604977TruSeq Adapter, Index 3 (97% over 36bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA527350.5033023160018928Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC507500.484357495725724No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG479030.4571857560147657No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT464130.4429652108200597No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC438580.4185803162076612No Hit
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGA425820.40640218488655727TruSeq Adapter, Index 3 (97% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA374990.35789008339347633Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC340330.3248106138331737Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACCCCTCATCCGCTACTTTGACCTCATTAGATCGGAAGAGCACACGTCT337440.3220524007048046Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTGTAACTCCCTAAGGAAGGAGATCGGAAGAGCACACGTCTGAACTCC326310.3114299397640611Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT261300.24938446036085063No Hit
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG226100.2157896153371157Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA223380.21319365003982707TruSeq Adapter, Index 3 (97% over 36bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG222800.21264009861614053No Hit
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT209230.19968890409988818Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT190400.18171757081020268Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC184700.17627749647397287Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA180950.17269850020013747No Hit
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC177520.16942491160833603Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG176970.16889999215484017Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA164220.1567314048237998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC159830.1525415931858965Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTGGGGGGGGGGT159660.15237934535481595TruSeq Adapter, Index 3 (96% over 32bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTAGATCGGAA149880.14304532307265325No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA137190.13093399968199423No Hit
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA135810.12961692905322283Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT135440.12926380142087107Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC133270.12719275557707832Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC124960.11926169983425908No Hit
TGTGCGTGTTTTCACTGCGGCGACTGTCGCAGATCGGAAGAGCACACGTC122080.11651303069595348No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAGATCGGAAGAGCACACGTCT111900.1067972488112483Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTAGTCGTGGCCGAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC109950.1049361707488539Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG109650.10464985104694709No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT107880.10296056480569678Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAAGAGGACTGATAATCTCAGGTTATCAGATCGGAAGAGCACACGTCTG105930.10109948674330237Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTTTTGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA104780.10000192788599284TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)