FastQCFastQC Report
Wed 18 Dec 2024
1B11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998647
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA6064026.7388130682312575Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1688051.8758931203768745Illumina RNA PCR Primer (100% over 37bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1649231.8327533016907986No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1409621.566479938595213Illumina RNA PCR Primer (100% over 45bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1026941.141216007250868Illumina RNA PCR Primer (100% over 36bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC964281.0715833169141982No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT793630.8819436966468404No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG724900.8055655477984635Illumina Small RNA Adapter 1 (100% over 26bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG330650.3674441279894633Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT321730.35753152668395594Illumina DpnII expression Sequencing Primer (100% over 22bp)
AATCTAGCGCGTCTACCAATTCCGCCAGTTCCGCGATCGTCGGACTGTAG299490.3328167001105833No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT280950.31221360277828436Illumina RNA PCR Primer (100% over 50bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA154220.17138131988064428No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT148660.16520261323730112No Hit
AATCTAGCGCGTCTACCAATTCCGCCACATCCGCGATCGTCGGACTGTAG145430.16161318473766112No Hit
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT144580.16066859829038743Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATGAGGTCAAAGTAGCGGATGAGGGGTGGATCGTCGGACTGTAGAACTC140510.15614569612520637Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG133150.14796668876998953Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCGTGAGCCTAGAGATCCACCGTCAAAAGTTGATCGTCGGACTGTAGAA115240.12806369668684636No Hit
GGTGCTCTAACCAGTTGAACTACGGTCCCGATCGTCGGACTGTAGAACTC113360.12597449372111164Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT110100.12235172687627373Illumina RNA PCR Primer (100% over 31bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC110000.12224059905894741Illumina RNA PCR Primer (100% over 32bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC106170.11798440365534953Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC101110.11236133609863794No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG95450.10607150163796845No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)