FastQCFastQC Report
Wed 18 Dec 2024
1B11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998647
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC5714346.350221316604595Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1632881.814583903557946No Hit
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCAT1578821.7545082055113397TruSeq Adapter, Index 12 (97% over 37bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCA968581.0763618130592298TruSeq Adapter, Index 12 (97% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT946581.0519136932474404No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG781570.8685416818772866No Hit
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC688300.7648927666570319Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTAGGGGG637180.7080842264398192TruSeq Adapter, Index 12 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTGGGGGG551560.6129365892450276TruSeq Adapter, Index 12 (97% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG307780.34202919616693483Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA306570.34068454957728644Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGAACTGGCGGAATTGGTAGACGCGCTAGATTAGATCGGAAGAGCACA293820.3265157528681812No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG150130.16683619215199796No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC144190.1602351998028148No Hit
GCGGATGTGGCGGAATTGGTAGACGCGCTAGATTAGATCGGAAGAGCACA142300.15813488405534742No Hit
CACCCCTCATCCGCTACTTTGACCTCATTAGATCGGAAGAGCACACGTCT136960.15220065861012216Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC133940.14884459852686743Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA126910.14103231296882743Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCAAGATCGGAAGAGCACACG111220.12359635843032847No Hit
GGGACCGTAGTTCAACTGGTTAGAGCACCAGATCGGAAGAGCACACGTCT109260.12141825321073269Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA102940.11439497515570952TruSeq Adapter, Index 7 (100% over 35bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAT102290.11367264434308846TruSeq Adapter, Index 9 (97% over 36bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT99290.110338809823299Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT98120.10903861436058108No Hit
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC94620.10514914075416004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)