FastQCFastQC Report
Fri 20 Dec 2024
1B10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10972576
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3855903.514124668628406No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1710861.5592145363130772No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1201851.0953216455279051Illumina RNA PCR Primer (100% over 50bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA1146111.044522270795846No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG889050.8102472928872855Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC833520.7596393043894158No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA736650.671355568646779Illumina RNA PCR Primer (100% over 45bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA655240.5971615051925819Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC494580.45074192240728156No Hit
GGCGAGAATTCTACCATTGAACCACCAGTGCGATCGTCGGACTGTAGAAC484240.44131842878099No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT404530.3686736824607093No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT376490.34311906338128806No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT320010.29164528001446516Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA313900.2860768519625656Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC306360.2792051747921363No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT302260.27546858641033795Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG301450.27473038236417774No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC272420.24827351389500513No Hit
CAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGA262800.23950620164307815Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT249850.22770405053471493No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG249300.22720280087374195Illumina DpnII expression Sequencing Primer (100% over 23bp)
GATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGA238330.21720514854488135Illumina DpnII expression Sequencing Primer (100% over 24bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT233060.21240226542974047Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC219670.20019911459259887No Hit
GGCGAGAATTCTACCATTGAACCACAGTGCGATCGTCGGACTGTAGAACT212070.19327275564097254No Hit
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC204240.18613678319475754No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT202100.18418646633206276No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCAGATCGTCGGACTG195910.17854512923856714No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG192370.17531890414794118No Hit
AACTGAATTTAAGGGTGATAACCCTCCCCAAGTCCGGACCTCGATCGTCG187490.17087145261058112No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT184690.16831963615471882Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT182630.1664422283336201Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA173370.1580030067688754No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA170780.15564257654720276No Hit
AACTGAAATTAAGAGTGGATAACCCTCCCCAGGTCCGGACCTCGATCGTC168480.15354644160131586No Hit
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG165050.1504204664428845No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG164070.1495273306833327No Hit
GGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTG153770.14014029157783914Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT149420.13617586244105304No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA143260.13056186623815594Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGTAAAGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAAC141470.12893052643244393No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT139790.12739943655892655No Hit
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT137970.12574075586261604No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG136300.12421877961929814No Hit
GATCGATGCGCTCGACACCCGAAATCATATGGATTCGATCGTCGGACTGT135210.12322539392755173No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG135060.12308868947455913Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCATCAAAGCTAAGACAGGTAACTAAGTGTCATCACCACTGATCGTCGG132930.12114748624206385No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA132760.12099255452867222Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAACCGGTGTTTTCCCTGTGGTATGGTCGTAGACGATCGTCGGACTGTA130730.11914248759817203No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC129760.11825846546881973Illumina DpnII expression Sequencing Primer (100% over 21bp)
TAAGAGGTCAATGTGCGGGGAGGGGTGGTAATGATCGTCGGACTGTAGAA120680.10998328924766618No Hit
TAAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACT117170.10678440504763877No Hit
AGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAA116870.10651099614165352No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACTC116670.10632872353766336Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA115830.10556317860090465No Hit
AGTGGATAACCCTCCCCAGGTCCGGACCTCGATCGTCGGACTGTAGAACT113770.10368577077980595No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGTAATGATCGTCGGACTGTAGAAC110110.1003501821267859No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)