FastQCFastQC Report
Fri 20 Dec 2024
1B10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10972576
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3755343.4224780033421505No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1643431.4977613278777928No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC1118961.019778764804181No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA836930.7627470522874482Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT814700.7424874523539413No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC609410.5553937379882354Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG486190.4430955866698941No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT469640.4280125286897079No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC385270.35112083069645633No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC364410.332109798100282No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTAGGGGG301930.27516783661375416TruSeq Adapter, Index 1 (97% over 37bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG301050.27436583715619745Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT293880.26783136430314997No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC293870.26782225067295046No Hit
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC291080.26527954784728763Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG285900.26055868740394234Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT261160.23801156629035877No Hit
AAAGGATTCTAATAATGGTAGTTTTGAGATCGGAAGAGCACACGTCTGAA241910.2204678281563053Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC239450.21822587512722624No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA234600.21380576448046476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGTCCGGACTTGGGGAGGGTTATCAGATCGGAAGAGCACACGTCTGAA222990.2032248398188356Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC213720.1947765046238914Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT210660.1919877337828419No Hit
GCACTGTGGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC203200.1851889656540087No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT201260.18342092139530405No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC192660.17558319942372694No Hit
TGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGC191950.17493613167956185No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA190910.17398831413881297No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACCCTTAAATTCAGTTAGATCGGA183660.16738093224416947No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG171930.1566906440201462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG171120.15595243997398606Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC169350.15433932742867307No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC164680.15008326212550271No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACTCTTAATTTCAGTTAGATCGG164180.1496275806155273No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAGATCGGAAGAGCACA159420.14528949264056135No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA151210.13780720224676501No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCAGATCGGAAGAGCACACGTCTGA145070.13221143330426693Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC139480.1271169140227418No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACCAGATCGGAAGAGCACACGT137290.12512102900904948No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA135860.12381777989051979No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTAGGGTG135260.12327096207854929TruSeq Adapter, Index 1 (97% over 37bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC132890.1211110317212658Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC132510.1207647137736845No Hit
GAATCCATATGATTTCGGGTGTCGAGCGCATCGATCAGATCGGAAGAGCA131630.11996271431612777No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC131170.11954348732695037No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTAGATCGGAA130840.11924273753036663No Hit
GTCTACGACCATACCACAGGGAAAACACCGGTTCTAGATCGGAAGAGCAC127730.11640839853831954No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA127540.11623523956452889Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT123200.11227992405794228Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT120820.11011088007045931Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTCAGATCGGAAGAGCAC116050.10576367846529383No Hit
ATTACCACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACG114870.10468827010175186No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTAGATCGGAAGAGCACACG112590.10261036241626398No Hit
TACCACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCT111250.10138913596952985Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTC110200.10043220479858148No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)