FastQCFastQC Report
Fri 20 Dec 2024
1A9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18212437
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA13261447.28152964921718Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA4514802.4789653356110444Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2455021.3479909360839517Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1668240.9159894417205121Illumina RNA PCR Primer (100% over 45bp)
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC1469090.806641088175075No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1096250.6019238391874739No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT944740.5187334347402273Illumina RNA PCR Primer (100% over 50bp)
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT873020.4793537515050841No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA857210.4706728704126746Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG830050.45575998423494885No Hit
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC815500.44777093806831014Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC634810.3485585152607528Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTTCATTTGTATCCTCACGGTTGACCCCTGATCGTCGGACTGTAGAACTC589060.3234383185512186Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTATTTTCAGTAAGGACCAACTAAGCCAGATCGTCGGACTGTAGAACTC553090.30368807864647657Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA519000.2849701003770116No Hit
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT513700.28206000108607104No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT493060.27072708611154017No Hit
TTTTTTTTTCCATCGCGAGTAAACAATTAAGATCGTCGGACTGTAGAACT433880.23823280761383006No Hit
ACAGACCCAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG422110.2317701908865903No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT411980.22620805771352842Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT403320.22145306528719905No Hit
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG388980.2135793249415221Illumina RNA PCR Primer (100% over 33bp)
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT381710.2095875472348923No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC374100.20540908391337193Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT370800.203597135298258No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGGATCGTCGGACT369870.20308649523399863No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG365610.20074743429448785Illumina DpnII expression Sequencing Primer (100% over 23bp)
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG353620.1941640209929072No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGATCGTCGGAC337470.18529645428560715No Hit
GCCTATTCAGAGAATTAAGACATATCGTAGATCGTCGGACTGTAGAACTC334780.1838194416266203Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC317320.1742325862266538No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT316890.17399648383135108Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTGTTCTCGCTGTTATTATATTCAACCAAGATCGTCGGACTGTAGAACT283460.15564089528490888No Hit
GCTCCAGATTGAGACAACAGATACGTAAGATCGTCGGACTGTAGAACTCT267860.14707532001346113Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT251410.13804303070478707Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTTTTTTTTTTTTTTCAGCGGTTAACCTTTCCTTTGATCGTCGGACTGTA240340.13196476671408663No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTAGATCGTCGGACTG239290.13138823760927767No Hit
TTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAACT230850.12675404175728927No Hit
TTTTTTTTTTTTTTTTCAGCGGTTAACCTTTCCTTTGATCGTCGGACTGT223270.12259205069590631No Hit
CAGGCTGATCCGAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTA220180.12089540790175418Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTACTTCAGATGTAGTATCGCCAAATCGTAGATCGTCGGACTGTAGAAC217750.11956115483062481No Hit
GCTGCTTTGAATCCACATTATCCTGGAGAAGATCGTCGGACTGTAGAACT217380.11935799695559689No Hit
TATTCTATCTAGTTGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT205110.11262084255940048Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTTTTCGAATTTTTCAAAGGTAGATGTAAGATCGTCGGACTGTAGAACT202090.11096263503890226No Hit
GGTGATCAGAGTAAGCCACCTCCATCAGCAGATCGTCGGACTGTAGAACT198120.10878280594738639No Hit
AGGCTGATCCGAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG197200.10827765663650613Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC196350.10781094259927981No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG195930.10758033095735622No Hit
GCTTCCTATGAGGTCACATGAGAACCAATCAGATCGTCGGACTGTAGAAC194890.10700929260592637No Hit
TTTCATTTGTATCCTCACGGTTAACCCCTGATCGTCGGACTGTAGAACTC190710.1047141576934487Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTCGTCGTATGTCCCTTAAATTCCATAAGATCGTCGGACTGTAGAACTC185950.10210055908498132Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCCACCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA183790.10091455635508856Illumina DpnII expression Sequencing Primer (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)