FastQCFastQC Report
Fri 20 Dec 2024
1A9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18212437
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC12286176.74603294441046Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC4196832.304375850414747Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC2267241.2448855691305891Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGG1495710.8212574736703275TruSeq Adapter, Index 3 (97% over 37bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT1375110.7550389879179815No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1045420.57401433976134No Hit
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA834580.4582472955156962No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA813490.4466672966391043Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC784880.4309582512214044No Hit
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT760710.4176871003040395Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAAATCTTTCTTCTGAGGCGGAAGATCGGAAGAGCACACGTCTGAACT580920.3189688453006042Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGGTCAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT553210.30375396768702617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCTTAGTTGGTCCTTACTGAAAATAGCAGATCGGAAGAGCACACGTCT515350.282965975393628Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG493440.2709357347399472No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC479350.2631992632287486No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC463380.2544305300822729No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGGG438040.24051696101954945TruSeq Adapter, Index 3 (96% over 33bp)
TTAATTGTTTACTCGCGATGGAAAAAAAAAAGATCGGAAGAGCACACGTC424530.23309895320434054No Hit
GATACGATCCACTGAGATTCATCCTGTGTTTTTGGGTCTGTAGATCGGAA409850.22503852724377305No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA387130.21256353556638247No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG386410.2121682013230849Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC381680.20957107497475488No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA358790.19700274048991906No Hit
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAG358070.19660740624662149TruSeq Adapter, Index 3 (97% over 37bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT352620.1936149456549939Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAG351260.19286820319543177No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC349290.19178652477974256No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA340280.18683935598514356Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGA325270.17859773516306468No Hit
TACGATATGTCTTAATTCTCTGAATAGGCAGATCGGAAGAGCACACGTCT316320.17368351088874048Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT299350.1643657024043515No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC289400.15890240279211398Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGGAAAGGTTAACCGCTGAAAAAAAAAAAAAAAAGATCGGAAGAGCAC279090.15324143605822768No Hit
AAAGGAAAGGTTAACCGCTGAAAAAAAAAAAAAAAAAGATCGGAAGAGCA272290.14950772376041713No Hit
TTGGTTGAATATAATAACAGCGAGAACAGTAGATCGGAAGAGCACACGTC266200.14616385495252504No Hit
TATGATTGAAATCTTCATGAACGACACAAAAGGCCAAAAAAAAAAAAAAA263750.14481862037463739No Hit
TTACGTATCTGTTGTCTCAATCTGGAGCAGATCGGAAGAGCACACGTCTG250560.13757631666756073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG232660.12774786811891237Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGC228420.1254197886861599No Hit
TTAATTGTTTACTCGCGATGGAAAAAAAAAAAAAAAAAGATCGGAAGAGC228030.1252056493043737No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAGATCGGAAGAGCACACGTC215270.11819944799259978No Hit
TACGATTTGGCGATACTACATCTGAAGTAGCAGATCGGAAGAGCACACGT205120.11262633331277962No Hit
AGTGATTGATAGGCGGGATGGATTCTTGAAAAAAAAAAAAAAAAGATCGG203800.11190155386673405No Hit
TTCTCCAGGATAATGTGGATTCAAAGCAGCAGATCGGAAGAGCACACGTC203220.1115830901707443No Hit
AGTGATTGATAGGCGGGATGGATTCTTGAAAAAAAAAAAAAAAGATCGGA201510.11064417134291253No Hit
CGGACCTCGGATCAGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT198410.10894203779538125Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTAATTGTTTACTCGCGATGGAAAAAAAAAAAAAAAAAAGATCGGAAGAG197930.10867848163318286No Hit
TTACATCTACCTTTGAAAAATTCGAAAAGTAGATCGGAAGAGCACACGTC192100.10547737241314821No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA191560.10518087173067503No Hit
TGCTGATGGAGGTGGCTTACTCTGATCACCAGATCGGAAGAGCACACGTC189260.1039179984534744No Hit
ACAACTAGATAGAATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC188070.10326459880135755Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT184540.10132636285852355No Hit
CGGACCTCGGATCAGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC182430.10016781389552644Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)