FastQCFastQC Report
Fri 20 Dec 2024
1A8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10539495
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC7591087.202508279571269No Hit
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2346492.226378019060686Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1580171.4992843585010478No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1453191.3788042026681544Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1167991.1082030021362503Illumina RNA PCR Primer (100% over 45bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA748790.7104609850851488Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT640280.607505388066506No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC595510.565027071980204No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT548850.520755501093743Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT511710.48551662105252674Illumina RNA PCR Primer (100% over 50bp)
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA508440.4824140056046328Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG456910.4335217199685564No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA401500.38094804352580464No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT354810.33664800827743646Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT334140.31703606292331843No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG299200.2838845694219695Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT281910.26747960884273864No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC270270.2564354364227128No Hit
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT266370.2527350693747661No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT253950.24095082354515088Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCGTCGTATGTCCCTTAAATTCCATAAGATCGTCGGACTGTAGAACTC248660.23593160772883331Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG232720.22080754343543024No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT221460.21012391959956336Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTTGGTCGACAGGTTCTGCACATAAAAGAGATCGTCGGACTGTAGAACT204790.19430722249974974No Hit
GCCGGATCCTAACCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT192000.1821719162066114No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG190920.18114719917794925No Hit
CATCAAGTTTTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG190720.180957436765234Illumina RNA PCR Primer (100% over 33bp)
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT186910.17734246280300905Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA182180.17285458174229412No Hit
GCTCCAGATTGAGACAACAGATACGTAAGATCGTCGGACTGTAGAACTCT179900.1706912902373406Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC175470.16648805279569848No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC174930.16597569428136738Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG170010.16130753892857297No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT160430.1522179193595139No Hit
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC159100.15095599931495768Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG158780.15065237945461335Illumina RNA PCR Primer (100% over 33bp)
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT156650.14863140975919623No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT152670.14485513774616335No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGGATCGTCGGACT148010.14043367352989872No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA146410.13891557422817696Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCGGGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTAGAA139120.13199873428470718No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT135960.1290004881638067No Hit
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC134320.12744443637954192Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC132790.12599275392227047No Hit
ACTTGGTTGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT125420.1190000090137146No Hit
GATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGA123730.11739651662627099Illumina DpnII expression Sequencing Primer (100% over 24bp)
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG123410.11709289676592664No Hit
TTTTTTTGGGCTTTTACCTACAGCATAAAGAAGATCGTCGGACTGTAGAA117220.11121975009239057No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA112920.10713985821901334No Hit
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT110480.10482475678388765No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA110180.10454011316481482No Hit
CTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACTC105820.10040329256762304Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)