FastQCFastQC Report
Fri 20 Dec 2024
1A8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10539495
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT7676167.283233209940325No Hit
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC2304482.1865184242698534Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1578611.497804211681869No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC1430001.3568012509138245Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGG1058371.0041942237270383TruSeq Adapter, Index 9 (97% over 45bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC737300.6995591344746593Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC643330.6103992648604131No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT580060.5503679255979532No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG547160.5191520087062995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC498580.4730587186577725Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC451210.42811349120617265No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG398070.3776936181477386No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG353760.33565175561068156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC332720.31568874979304035No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA293800.27876098427865853Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC278730.2644623864805667No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT269950.2561318165623685No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC264510.2509702789365145No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC248870.2361308582621843Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTATGGAATTTAAGGGACATACGACGAGTAGATCGGAAGAGCACACGTCT245730.2331515883825553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA231640.21978282640676808No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG217820.20667024368814635Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTTTTATGTGCAGAACCTGTCGACCAAGTAGATCGGAAGAGCACACGTC202810.19242857461386909No Hit
TCCCATATGGTCTAGTGGTTAGGATCCGGCAGATCGGAAGAGCACACGTC193300.18340537188926034No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGGGGGGG192290.18244707170504848TruSeq Adapter, Index 9 (97% over 41bp)
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA188940.17926855129206856No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG186770.1772096291141084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAAACTTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC185880.17636518637752568TruSeq Adapter, Index 9 (100% over 37bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC180850.1715926616977379No Hit
TTACGTATCTGTTGTCTCAATCTGGAGCAGATCGGAAGAGCACACGTCTG179210.17003660991347308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT174080.16516920402732768Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT173500.16461889303045354No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA169270.16060541800152664No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC158840.1507093081784279No Hit
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT157700.14962766242595113Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC156160.14816649184804395TruSeq Adapter, Index 9 (100% over 37bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC154850.14692354804475927No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC151200.1434603840127065No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA146670.13916226536470677Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAG146020.13854553752338228No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC135360.12843120092566107No Hit
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT134320.12744443637954192Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGTAGTATAGTGGTGAGTATCCCCGCCAGATCGGAAGAGCACACG132990.1261825163349857No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG131160.12444619025864144No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGTAGATCGGAAGAGCACACGTC124190.117832970175516No Hit
GAGGTCCGGACTTGGGGAGGGTTATCAGATCGGAAGAGCACACGTCTGAA123080.11677978878494653Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAGATCGGAAGAGCACA122570.11629589463252271No Hit
TTCTTTATGCTGTAGGTAAAAGCCCAAAAAAAAGATCGGAAGAGCACACG116810.11083073714632437No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG113510.10769965733652324No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC110500.10484373302515919No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC109590.10398031404730491No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)