FastQCFastQC Report
Fri 20 Dec 2024
1A2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16221488
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG6165023.8005268074050913Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3748742.3109717185007934No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2314611.4268789644945026Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1841761.135382894590188Illumina RNA PCR Primer (100% over 50bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1732931.0682928717760047No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1322000.8149683925420405No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC960760.5922761216480263No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA953890.5880409984583412Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC845550.52125304411038No Hit
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG839400.517461776626164Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT811770.5004288139287838Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG760580.4688719062024396No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA748670.46152979307447006No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA736140.45380547086679107Illumina RNA PCR Primer (100% over 45bp)
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG686460.4231794271894169No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT611830.3771725503850202No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT541120.33358222131040016Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA521090.32123440217075033No Hit
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC515490.31778219112821215No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC511180.31512522155797296No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG498550.3073392527245343No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA455430.2807572276969906Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAGATCGT432050.2663442465943938No Hit
TGAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTC398970.2459515427931149Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC384230.23686483015614843Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA340810.2100978652513259No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT329470.20310713789018614No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT328580.20255848292092563No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT297420.18334939433423122Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT285240.17584083531671077No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACTC260850.16080522329394198Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGGAT259260.15982504194436417No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC258220.15918391703646423No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT253520.15628652562576256Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT250280.15428917495115121Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG249470.15378983728249837Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT238600.1470888490624288No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC230730.1422372596151475No Hit
AACTGAAATTAAGAGTGGATAACCCTCCCCAGGTCCGGACCTCGATCGTC229560.1415159940937601No Hit
AACTGAATTTAAGGGTGATAACCCTCCCCAAGTCCGGACCTCGATCGTCG223640.13786651384879117No Hit
TAAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACT222810.13735484685498645No Hit
ACTCGTCAAGACCAGAAGAACACACAACAGATCGTCGGACTGTAGAACTC221810.13673838059739032Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC217730.13422319826639825No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT209810.12934078550623715No Hit
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC195640.12060545863610048No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA189290.11669089790036524No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA174070.10730828145975266Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAATCTCAGCCGATCGTAACGACAGATCGTCGGACTGTAGAACTCTGAAC163530.10081072710468979Illumina DpnII expression Sequencing Primer (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)