FastQCFastQC Report
Fri 20 Dec 2024
1A2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16221488
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA6070693.7423755453260514Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3792122.337714024755312No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC2279891.4054752560307662Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1741511.0735821522661793No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1326650.8178349606398624No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA952030.5868943712192124No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC939960.5794536234900276Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGGGGGGG902810.5565519020203326TruSeq Adapter, Index 9 (97% over 41bp)
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA841710.518885813681211Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG835760.5152178394485142No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG801110.4938572836228094Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC750980.4629538301295171No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC745430.45953244239985874No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA698960.43088525540936806No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGG676640.4171257285398232TruSeq Adapter, Index 9 (97% over 45bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC611760.37712939774698845No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC528490.3257962524769614Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC515260.31764040388896503No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT509450.3140587349323318No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT507740.3130045776318424No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA494800.3050275042585489No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC445520.27464804708421325Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCG428400.26409414475416804No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAAGATCGGAAGAGCACACGTCT392380.24188903015555666Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT379940.2342201899110612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC337890.20829778377914526No Hit
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA326590.20133171506830938No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC326030.2009864939640556No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG295850.18238154230980536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC283880.1750024412063801No Hit
TACCACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCT259650.16006546378482664Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGA258070.1590914470978248No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT257940.15901130648433734No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG250500.15442479752782234Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG249600.1538699778959859Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGTTTGGTCTAGTGGTATGATTCAAGATCGGAAGAGCACACGTCTGA246560.15199592047289373Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC237410.14635525421588946No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT228610.1409303511490438No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACTCTTAATTTCAGTTAGATCGG224340.13829804022910847No Hit
TACCACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTC221110.13630685421707306No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACCCTTAAATTCAGTTAGATCGGA219940.13558558869568563No Hit
TGTTGTGTGTTCTTCTGGTCTTGACGAGTAGATCGGAAGAGCACACGTCT219190.13512323900248857Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT215760.13300875973893395No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC208240.12837293348181128No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT195630.12059929397352451No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGGGGGGT193350.11919375090620539TruSeq Adapter, Index 9 (97% over 41bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG188530.1162223835445922No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT170900.10535408342317301Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTCGTTACGATCGGCTGAGATTCAGATCGGAAGAGCACACGTCTGAACT162890.10041618869982827Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)