FastQCFastQC Report
Fri 20 Dec 2024
1A1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13405128
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2325861.7350524366496165No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1953141.4570095861822432Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1393781.03973643519107Illumina RNA PCR Primer (100% over 50bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG1023220.7633049083902818Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT893660.6666553277223463No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG872740.6510493596181999No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA814640.6077077369197817Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC694580.518144996452104No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA653200.48727621250613945No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT647720.4831882246853592Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC641430.47849599048960967No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG598130.44619491884001405No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA554890.4139386061811569Illumina RNA PCR Primer (100% over 45bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC529600.395072691584892No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC516900.3855987052119159No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG510460.38079457353932017No Hit
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC455030.33944472592876396No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT433120.3231002344774328Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA416770.3109034095011998No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA405240.3023022234476239Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA357740.26686802244633545No Hit
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT344300.25684200852091826No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT295870.22071404316318352Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC271550.20257173225052386No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC262720.19598470078017902No Hit
TGCTCAGGAAGAAGTACAGAGTACCGATGATCGTCGGACTGTAGAACTCT261590.19514173978793786Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT248460.18534698064800276No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT239850.17892406547703238Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACT221630.1653322519561171No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGGAT218960.16334047686825517No Hit
ACTGTTCTTGCTGTTACTATATTCAACCAAGATCGTCGGACTGTAGAACT215390.16067731691931625No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT212150.1582603314194389No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA210860.1572980131185618Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT205030.1529489311851405No Hit
ACTGTTCTCGCTGTTATTACATTCAACCAAGATCGTCGGACTGTAGAACT205030.1529489311851405No Hit
ACTGTTCTCGCTGTTATTATATTCAACCAAGATCGTCGGACTGTAGAACT200450.1495323282254373No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT198780.1482865363165499No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC188770.14081924469501522No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT188140.1403492752922613Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA181130.1351199332076501Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC170650.1273020294919974Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC167880.12523565608623805No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAACTC167300.12480298584243282Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA162990.12158779834105277No Hit
TTTTTTTTTCCATCGCGAGTAAACAATTAAGATCGTCGGACTGTAGAACT162650.12133416406020144No Hit
GCTTCCTATGAGGTCACATGAGAACCAATCAGATCGTCGGACTGTAGAAC162320.12108798961113987No Hit
ACTTGGTCGACAGGTTCTGCACATAAAAGAGATCGTCGGACTGTAGAACT159260.1188052810834779No Hit
TTTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGA159240.11879036141989842No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG155650.11611228180737998Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG152330.11363561765318467No Hit
ACTGTTCTCGCTGTTACTATATTCAACCGAGATCGTCGGACTGTAGAACT151600.11309104993253327No Hit
CTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACTC150940.11259870103441012Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACTC150480.11225554877208185Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT144450.10775727020286566No Hit
GGAGTTGCTGAGCAATACCTTGTGACTGAGATCGTCGGACTGTAGAACTC143480.1070336665192604Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTTTTTTTTTTTTTCAGCGGTTAACCTTTCCTTTGATCGTCGGACTGTA141890.10584755326469093No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)