FastQCFastQC Report
Fri 20 Dec 2024
1A1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13405128
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2300821.7163730178480952No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC1904681.4208592413291392Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGGGGGGG1165510.8694508549265625TruSeq Adapter, Index 1 (97% over 41bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA985540.7351962622065228Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC874230.6521608745548718No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC853580.6367563219090485No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC791990.5908112179160094Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT683490.5098720429972768No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC634690.4734680638633216No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC627610.46818650295618214Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA625120.46632900484053563No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA618610.46147265434541174No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGG528740.39443114605097396TruSeq Adapter, Index 1 (97% over 45bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG509480.3800635100239252No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA503080.3752892176784884No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT450490.33605796229621976No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT445500.33233550623313707No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG416200.3104781990891844No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG394880.29457383771344814Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC393400.29346978260856593Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC349120.26043764744357534No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC329830.24604763192115736No Hit
TATGATTGAAATCTTCATGAACGACACAAAAGGCCAAAAAAAAAAAAAAA321630.23993056985356648No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG290240.2165141578655571Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT265050.1977228415871896No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT263510.19657402749156888No Hit
ATCGGTACTCTGTACTTCTTCCTGAGCAAGATCGGAAGAGCACACGTCTG256910.19165053851033723Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC244580.18245256591358172No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG235680.17581331562070873Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGA217390.16216928327726524No Hit
TACCACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTC215800.1609831700226958No Hit
TTGGTTGAATATAGTAACAGCAAGAACAGTAGATCGGAAGAGCACACGTC209370.1561864981818898No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT206630.15414250427149967Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC204250.1523670643055404No Hit
TTGGTTGAATGTAATAACAGCGAGAACAGTAGATCGGAAGAGCACACGTC199560.14886840319615002No Hit
TTGGTTGAATATAATAACAGCGAGAACAGTAGATCGGAAGAGCACACGTC195750.1460262072842572No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT183740.1370669493047735No Hit
AAAGGAAAGGTTAACCGCTGAAAAAAAAAAAAAAAAGATCGGAAGAGCAC181860.13566450092830148No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG181690.1355376837878758Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA178440.13311323845620868Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC177010.13204648251027518No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC173220.12921920626196184No Hit
TTAATTGTTTACTCGCGATGGAAAAAAAAAAGATCGGAAGAGCACACGTC168880.12598163926521255No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAGATCGGAAGAGCACACGTCT165160.12320658183942743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAAGATCGGAAGAGCACAC165030.12310960402616072No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT164110.1224232995015042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG161550.1205135825633295No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGTAGATCGGAAGAGCACACGTC155690.11614212113453896No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT153890.11479935141238487No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA152650.11387433227045649Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATTGGTTCTCATGTGACCTCATAGGAAGCAGATCGGAAGAGCACACGT152120.11347896118560001No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA149930.11184525802364588No Hit
TCGGTTGAATATAGTAACAGCGAGAACAGTAGATCGGAAGAGCACACGTC148340.11065914476907643No Hit
TACCACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCT146250.10910003992501974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTTTATGAGTGGAACCTCTCGACCAAGAGATCGGAAGAGCACACGTCT144950.10813026179235288Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA142680.1064368799760808No Hit
TCAGTCACAAGGTATTGCTCAGCAACTCCAGATCGGAAGAGCACACGTCT141380.10546710184341394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)