FastQCFastQC Report
Fri 20 Dec 2024
1A12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8194972
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2337932.852883450974573Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1369461.6710978390164115Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC818520.9988075615145482No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT814670.9941095588856191Illumina RNA PCR Primer (100% over 50bp)
CAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGA704440.8596002524474764Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT633940.7735718926190351Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC504650.6158044225166358Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA427140.5212220371222746Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT398720.48654223589781637Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT390430.4764262769903302No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT378420.4617709493089177Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT358560.4375365773061824No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC275670.33638919083555135No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC275280.3359132892705429No Hit
CATCAAGTTTTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG270750.33038550955390694Illumina RNA PCR Primer (100% over 33bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT259220.31631590687558175No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG238950.2915812280993761No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG233140.28449151504117404No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA220220.268725750374742Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC216480.2641619763923538Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG203940.2488599106866991No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC201080.24536996587663754No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGATCGTCGGAC188030.22944556735520266No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG179420.21893912511232494Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT161370.19691342447539784Illumina DpnII expression Sequencing Primer (100% over 22bp)
TATCAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTC156740.19126361871645198Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT154500.18853023536871144No Hit
GACAACGGTTCAGAGTTGAATTCAGACAGGAGATCGTCGGACTGTAGAAC154320.18831058849255372No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT153350.18712693588214824No Hit
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGAGATCGTCGGACTGTAGAAC148030.18063515043126444No Hit
ACTTGGTTGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT139310.1699944795418459No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC128330.15659602009622486No Hit
GATTTTCCTCAGGTTGAGCTTCGTCTTCAGATCGTCGGACTGTAGAACTC127540.15563201436197707Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAAATCCAGAGACTTCAAATCGTTAGCACGATCGTCGGACTGTAGAACTC127040.15502188415042784Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA127000.1549730737335039Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT124330.15171497840383102No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGGATCGTCGGA121580.14835926224031026No Hit
ACTCGTCGTATGTCCCTTAAATTCCATAAGATCGTCGGACTGTAGAACTC121540.14831045182338634Illumina DpnII expression Sequencing Primer (100% over 21bp)
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG117160.1429657111702151No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT116610.14229456793751094No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG113340.138304316353979No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT98960.1207569714698232Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTTGGTCGACAGGTTCTGCACATAAAAGAGATCGTCGGACTGTAGAACT98560.12026886730058382No Hit
GCTTGTCAGTGGTAACTCAGGCCTTAGAGATCGTCGGACTGTAGAACTCT97240.11865812354209385Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCATTGAACCACCAGTGCGATCGTCGGACTGTAGAAC97140.11853609749978401No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGGATCGTCGGACT95840.11694975894975601No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATGATCGTCGGACTGTAG92670.11308153340853391No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT90860.11087286204272571No Hit
TCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC90350.11025052922694549Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC88970.10856656984306963Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGAA88850.10842013859229782Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA88020.1074073224411261No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA87300.10652873493649521No Hit
ACATGAATGTTGTCAGTAGGTCAGATCAGAGATCGTCGGACTGTAGAACT86200.10518644847108691No Hit
ACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGAACGT85130.10388076981837156Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG83880.10235544428949848Illumina RNA PCR Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)