FastQCFastQC Report
Fri 20 Dec 2024
1A12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8194972
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC2193692.6768730875468516Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT800410.9767086452522351No Hit
AAAGGATTCTAATAATGGTAGTTTTGAGATCGGAAGAGCACACGTCTGAA659570.8048471672630486Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG605010.7382697585787968Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT482660.5889708958127008Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC402070.4906301083151962Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC374120.45652382948959436No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG373610.4559014966738141Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTAGGGGG359570.43876904033351183TruSeq Adapter, Index 6 (97% over 36bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC351770.4292510090333439Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC347720.42430895431979515No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT267070.32589495119690465No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT266950.3257485199461328No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC248980.30382044014305354No Hit
TAAAACTTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACG247880.30247815367764525TruSeq Adapter, Index 6 (97% over 36bp)
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA235700.28761538172430606No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA231740.2827831504488362No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA214280.26147740346153714Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT204970.2501167789224905Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA201470.2458458674416459No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT197450.24094042054079012No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGA185800.2267243866116931No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTGGGGGG177730.21687688499728858TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTAGGGTG173590.21182500684566097TruSeq Adapter, Index 6 (97% over 36bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA170670.20826184641021347Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG155220.18940882287334235Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTGTCTGAATTCAACTCTGAACCGTTGTCAGATCGGAAGAGCACACGT151710.18512570878826676No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC148500.1812086728301207No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTAGTGTG148450.1811476598089658TruSeq Adapter, Index 6 (97% over 36bp)
AAAGGATTCTAATAATGGTAGTTTTGATAAGATCGGAAGAGCACACGTCT147260.17969554990547862Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAAGGCCTGAGTTACTACTGACGAGCAGTAGATCGGAAGAGCACACGT145860.17798718531314078No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC144910.17682793791119725No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGTAGATCGGAAGAGCACACGTC132830.1620871920001679No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG126170.1539602575823322No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA126110.15388704195694627No Hit
TGAAGACGAAGCTCAACCTGAGGAAAATCAGATCGGAAGAGCACACGTCT122220.1491402289110933Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCTAACGATTTGAAGTCTCTGGATTTTAGATCGGAAGAGCACACGTCT122030.1489083794307046Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAG120010.1464434533760457No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC119790.14617499608296405Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC118860.1450401538894825No Hit
TTATGGAATTTAAGGGACATACGACGAGTAGATCGGAAGAGCACACGTCT116470.14212373147827717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAGATCGGAAGAGCACA113650.13868259708513953No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC110420.1347411559185315No Hit
TCCAAGACCTGAGTTACCACTGACAAGTAGATCGGAAGAGCACACGTCTG95690.11676671988629125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT94590.11542443342088295No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGTAGATCGGAAGAGCACACGTC94470.11527800217011114No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAG93800.11446042768663517No Hit
TCTAAGGCCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG92390.11273986049006636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAAGAGCACA90420.11033594745656239No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC88770.10832251775844993No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTAGTGGG86200.10518644847108691TruSeq Adapter, Index 6 (97% over 36bp)
TAGTAACAATGACCTGATCTGGAGACTCTAGATCGGAAGAGCACACGTCT86020.10496680159492919Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC85490.10432006357068699No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC85270.10405160627760535No Hit
AAAGGATTCTAATAATGGTAGTTTTAGATCGGAAGAGCACACGTCTGAAC85120.10386856721414058Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAGGTGGAAGATCGGAAGAGCACACGTCTGAACT84100.10262390158258015Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGATCTGACCTACTGACAACATTCATGTAGATCGGAAGAGCACACGTC83720.10216020262180273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)