FastQCFastQC Report
Wed 18 Dec 2024
1A11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3239089
Total Bases485.8 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2223816.865541514913606No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1756025.421339148137023No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT771092.380576760934942Illumina RNA PCR Primer (100% over 50bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA580241.7913678815247125Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA555461.714864889479727Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT498181.5380250434612943No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT400031.2350077444614829No Hit
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT375041.1578564219754381Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT338391.0447073235715352No Hit
ACTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT189620.585411515398311No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA183550.5666716783638857No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT163480.5047098119255136No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG157440.4860625935255252No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC120540.37214167316798025No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT114980.35497635291898433Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA89830.2773310643826088No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC82350.2542381515296431No Hit
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGG78910.24361788144753047No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT77420.23901782260382473Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC72660.22432233260648288No Hit
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT71950.22213035825813984Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAGACTGCAGACCTATAGCACAAGTTTGATCGTCGGACTGTAGAACTC69190.21360944388993325Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT65880.20339052122371445No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG65720.20289655517338362Illumina RNA PCR Primer (100% over 37bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG63910.19730856422901624Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACACTCTTCTTGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAACT61080.1885715397137899No Hit
AATATCAATTTATCAGTCTGGGATAGCCACAGATCGTCGGACTGTAGAAC59930.18502115872703714No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT57920.17881571021975623No Hit
AAGAATGGCGCAAGAATCCAAAGTGGTCAGATCGTCGGACTGTAGAACTC57050.1761297698210824Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA53930.16649743183963145No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50350.1554449414634794No Hit
ATTATTACCAATAGTCTCAAAAGTCACTGAGATCGTCGGACTGTAGAACT50250.15513621268202263No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGTGATCGTCGGACTGTAGAAC48810.15069051822904528No Hit
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC45550.14062595995355484Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT45110.1392675533151451Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGAGAATTCTACCACTGAACCACCAGTGTGATCGTCGGACTGTAGAAC44860.1384957313615032No Hit
AACACTACTTAGACCAACGACGTTTCCAGATCGTCGGACTGTAGAACTCT42590.1314875880224347Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCAGAGATTGGTGAGCTGAGAGATCGTCGGACTGTAGAACTCTGAACGT41910.12938823230852872Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT41870.12926474079594602No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC41260.12738149522905978No Hit
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT39720.12262707199462565Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA39370.12154652125952699No Hit
AATGGAAGCCATACAGGTTGTCACGTTGAAGATCGTCGGACTGTAGAACT39350.12148477550323564No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA39270.12123779247807023No Hit
AACACTCTTCTTGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAAC37610.11611289470588798No Hit
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAG37600.11608202182774231No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA36880.11385917460125362No Hit
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT36520.11274775098800928Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGTG34300.10589397203966917Illumina DpnII expression Sequencing Primer (96% over 29bp)
AATCAGAAACTAAGAGACAATCAACATCAAGTCGATCGTCGGACTGTAGA34010.10499865857344456No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGGATCGTCGG33500.10342414178801508No Hit
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA32640.10076907426748694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)