FastQCFastQC Report
Wed 18 Dec 2024
1A11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3239089
Total Bases485.8 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2246486.935530329669855No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1810535.589627206909102No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC524931.6206099925009778Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC503611.5547890162943965No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC411881.271592105064109No Hit
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC375551.1594309387608677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC332771.027356766053665No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGTAGATCGGAAGAGCACACGTC191860.5923270401029426No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG187970.5803174905042745No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC171970.5309208854711926No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC165850.5120266840460389No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGGG151070.4663965701467296TruSeq Adapter, Index 19 (97% over 43bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT118630.36624495344215613No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG114230.3526608870580586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG90400.27909081843691236No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT83810.25874559173891176No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTAGATCGGAA80080.24723000819057458No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG77120.23809163625945445Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGTGTG73580.2271626373958851TruSeq Adapter, Index 19 (97% over 43bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG73200.22598946802634937No Hit
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC72040.22240821416145096Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACTTGTGCTATAGGTCTGCAGTCTTGTAGATCGGAAGAGCACACGTCT70480.21759204517072545Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC65930.20354488561444284No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGTG65200.20129116550980847TruSeq Adapter, Index 19 (97% over 43bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA65040.20079719945947766Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAAT63830.19706158120385084TruSeq Adapter, Index 19 (97% over 41bp)
AGCTGTCGACTCCTGCACCAAGAAGAGTGTAGATCGGAAGAGCACACGTC62040.1915353360157748No Hit
TGTGGCTATCCCAGACTGATAAATTGATATTAGATCGGAAGAGCACACGT60670.18730575170981717No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG58450.18045197276147706No Hit
TGACCACTTTGGATTCTTGCGCCATTCTTAGATCGGAAGAGCACACGTCT57870.17866134582902787Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC54620.1686276604316831No Hit
TCAGTGACTTTTGAGACTATTGGTAATAATAGATCGGAAGAGCACACGTC50830.15692683961447185No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT48810.15069051822904528No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT47200.1457199848475914No Hit
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT46760.14436157820918166Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAGGTCTAGGCTGGTTAGTTCAGATCGGAAGAGCACACGTCTGAACTCC46220.14269444278931515Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTAGGGGG45550.14062595995355484TruSeq Adapter, Index 19 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTAGTGTG44980.1388662058992513TruSeq Adapter, Index 19 (97% over 43bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC42990.13272250314826176No Hit
TGGAAACGTCGTTGGTCTAAGTAGTGTTAGATCGGAAGAGCACACGTCTG42500.13120973211912362Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCAGCTCACCAATCTCTGCTAGATCGGAAGAGCACACGTCTGAACTCC42120.13003656274958791Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT42080.1299130712370052No Hit
TTCAACGTGACAACCTGTATGGCTTCCATTAGATCGGAAGAGCACACGTC40030.1235841312171416No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC39920.12324452955753916No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTAGATCGGAAGAGCACAC39650.12241096184760591No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG38410.11858272495754209No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGTTAGATCGGAAGAGCACACGT38110.11765653861317178No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCTAGATCGGAAGAGCACA38080.11756391997873478No Hit
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC37910.11703908105025827Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG36580.11293298825688335Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAGGTCTAGGCTGGTTAGTTAGATCGGAAGAGCACACGTCTGAACTCCA34480.10644968384629136Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAA34080.1052147687204643No Hit
GACTTGATGTTGATTGTCTCTTAGTTTCTGATTAGATCGGAAGAGCACAC34050.10512215008602727No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC33170.10240533680920777No Hit
TGATTAGATCTAATGGTTTTTGAGCAAATTAGATCGGAAGAGCACACGTC32510.10036772685159315No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)