FastQCFastQC Report
Fri 20 Dec 2024
1A10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8619516
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT3684354.274427937717153Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3364263.9030729799677846No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1770062.053549178399344Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG1457041.6903965373461802Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1307381.5167672987671232No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG1088241.2625302859232468Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT923651.0715798891724315No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT919401.0666492178911204Illumina RNA PCR Primer (100% over 50bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG617210.7160610874206859No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA470960.5463879874461629Illumina RNA PCR Primer (100% over 45bp)
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT465450.5399955171496869No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC432710.5020119459143646No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA383400.4448045574716724No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT363820.42208866483918583No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA361570.41947830945496245No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA345970.40137984545767996Illumina DpnII expression Sequencing Primer (100% over 25bp)
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT258490.29988922811907304No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT240490.2790063850452856No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC232770.27004996568252787No Hit
AAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT181010.21000019026590355Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG177950.20645010694335972Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT167910.1948021211399805Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG165240.1917044994173687Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA157160.18233042319313522No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT157010.18215639950085366Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA154370.17909358251669816Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG153940.17859471459882434No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG148720.17253869010742598No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT145730.1690698178412802No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCAGATCGTCGGACTG141350.16398832602665858No Hit
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA139960.16237570647818278No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT139200.16149398643728952Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC135450.15714339413025047No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC122890.142571810296541No Hit
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT120370.13964821226621077No Hit
GCCGGATCCTAACCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT119980.1391957506662787No Hit
ACCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT117570.13639977001028827No Hit
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA117550.1363765668513174No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG116610.1352860183796863No Hit
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC111040.12882393860629762No Hit
GCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTAG107090.12424131470954983Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC103880.12051720769472439Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC103190.1197166987102292No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT101940.11826650127454953Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC100660.11678149910041352Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTC96760.11225688310109291Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC93440.10840515871192767No Hit
AGCCGAGATATCCGTCGCTGAAAGTTGTGGATCGTCGGACTGTAGAACTC89340.10364851112289833Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG89150.103428081112675Illumina RNA PCR Primer (100% over 33bp)
GGCGGGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTAGAA86720.1006088972977137No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAACTC86420.10026084991315058Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)