FastQCFastQC Report
Fri 20 Dec 2024
1A10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8619516
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG3535014.10116994968163Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3310263.840424450746423No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC1678451.9472671087332514Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1397911.6217963978487886Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1289151.495617619365171No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA1040871.2075736039007294Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC904361.0492004423450225No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC599490.6955030885724905No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC458250.5316423799201718No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT420210.48750997155756776No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG373030.4327737195452738No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC357690.41497689661461273No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC354150.4108699374767678No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC327500.379951728148077Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA253550.29415804785326694No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC232990.2703052004312075No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT226340.2625901500733916No Hit
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA176330.20457065106671882Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTTAGATCGGAAGAGCACACGTCTG173230.20097416142623323Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA159030.184499918556912Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC157480.1827016737366692Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC153410.17797983088609615No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA151570.17584514026077566No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG149880.1738844733277367Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA146850.17036919474364917No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT144720.167898058313251Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC141960.16469602237527026No Hit
TGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGC138460.16063546955536714No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC136980.1589184357915224No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG133380.1547418671767649Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA132770.15403417082815324No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG120280.13954379805084183No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTAGTGTG120150.13939297751753116TruSeq Adapter, Index 25 (97% over 46bp)
TCCCATATGGTCTAGTGGTTAGGATCCGGCAGATCGGAAGAGCACACGTC118480.1374555137434631No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC116620.13529761995917172No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGTAGATCGGAAGAGCACACGTC115930.13449711097467654No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG114850.1332441403902493No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA114760.13313972617488037No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTAGTGGG110290.12795382014488982TruSeq Adapter, Index 25 (97% over 46bp)
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT107460.124670573150511No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT100870.1170251322696077Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCGCGCTAGTCCCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC100550.11665388172607372Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT100320.11638704539790866No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG96880.11239610205491816Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT95870.11122434252688897Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGTTTGGTCTAGTGGTATGATTCTCAAGATCGGAAGAGCACACGTCT92960.10784828289662667Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT91230.10584120964564599No Hit
CACAACTTTCAGCGACGGATATCTCGGCTAGATCGGAAGAGCACACGTCT87870.10194307893853902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)