FastQCFastQC Report
Fri 4 Oct 2024
10--1B2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename10--1B2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21439633
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220730.5693800822056982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGCTGCATCTGGGGG762010.3554211958758809TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGCTGCATCTAGGGG461000.21502233736930107TruSeq Adapter, Index 5 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)