---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
layout: post
title: Reverse Transcription - C.gigas PolyIC RNA
date: '2023-07-21 12:05'
tags: 
  - reverse transcription
  - cDNA
  - RNA
  - Crassostrea gigas
  - Pacific oyster
  - MMLV
  - oligodT
categories: 
  - 2023
  - Miscellaneous
---
Quantified RNA [earlier today]() and proceeded to make cDNA. Reverse transcription was performed using oligo dT primers using M-MLV RT (Promega), per the manufacturer's recommendations. Used 400ng of RNA in each reaction. I used 400ng (instead of the usual 100ng) to simplify pipetting for high concentration samples, without the need/time to dilute samples. All reactions were done on ice in 0.5uL PCR tubes.

cDNA was stored in the same -80<sup>o</sup>C box as the original RNA.

- [20230721-cgig-polyIC-cDNA-calcs](https://docs.google.com/spreadsheets/d/1YEChD7YUpG2kS5G9mGkBpmX0tVUVGfzLOWHgmLmROl0/edit?usp=sharing) (Google Sheet)