---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
layout: post
title: RNA Quantification - C.gigas RNA from Noah's Heat-Mechanical Stress Project
date: '2023-07-13 12:38'
tags: 
  - RNA quantification
  - qubit
  - Crassostrea gigas
  - Noah
  - Pacific oyster
categories: 
  - 2023
  - Miscellaneous
---
I quantified Noah's [_Crassostrea gigas_ (Pacific oyster)](http://en.wikipedia.org/wiki/Pacific_oyster) ctenidia RNA [from yesterday (20230712)](https://robertslab.github.io/sams-notebook/posts/2023/2023-07-12-RNA-Isolation---C.gigas-Ctenidia-from-Noahs-Heat-Mechanical-Stress-Project/) using the Roberts Lab Qubit 3 and the RNA High Sensitivity (HS) Assay. 1uL of sample was used for each measurement.


---

# RESULTS

[20230713-qubit-cgig-ctenidia-RNA](https://docs.google.com/spreadsheets/d/1i3OHh1rPBvj9pVOXjeoRbnofW7pdg4iZJXfwkgi_4pg/edit?usp=sharing) (Google Sheet)

Yields were good. Will proceed with reverse transcription.

| Sample         | Original sample conc. | Units |
|----------------|-----------------------|-------|
| NTC1_ctenidia  | 76.2                  | ng/µL |
| NTC2_ctenidia  | 50                    | ng/µL |
| NTH1_ctenidia  | 57.4                  | ng/µL |
| NTH2_ctenidia  | 47.8                  | ng/µL |
| NTHM1_ctenidia | 68.6                  | ng/µL |
| NTHM2_ctenidia | 32.6                  | ng/µL |
| NTM1_ctenidia  | 30.6                  | ng/µL |
| NTM2_ctendia   | 57                    | ng/µL |
| TC1_ctenidia   | 39.4                  | ng/µL |
| TC2_ctendia    | 76                    | ng/µL |
| TH1_ctenidia   | 88.6                  | ng/µL |
| TH2_ctenidia   | 29.6                  | ng/µL |
| THM1_ctenidia  | 31.2                  | ng/µL |
| THM2_ctenidia  | 59.2                  | ng/µL |
| TM1_ctendia    | 84                    | ng/µL |
| TM2_ctenidia   | 68.2                  | ng/µL |