---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
layout: post
title: Samples Submitted - M.magister MBD-BSseq Libraries to Univ. of Oregon GC3F
date: '2021-01-12 09:16'
tags:
  - Metacarcinus magister
  - sequencing
  - Dungeness crab
  - Cancer magister
categories:
  - 2021
  - Miscellaneous
---
Submitted the _M.magister_ MBD-BSseq libraries [created 20201124](https://robertslab.github.io/sams-notebook/posts/2020/2020-11-24-MBD-BSseq-Library-Prep-M.magister-MBD-selected-DNA-Using-Pico-Methyl-Seq-Kit/) using the [4nM aliquots created for the MiSeq test run on 20201202](https://robertslab.github.io/sams-notebook/2020/12/02/Sample-Submission-M.magister-MBD-BSseq-Libraries-for-MiSeq-at-NOAA/) to the Univ. of Oregon GC3F sequencing core.

For library pooling, I used an updated set of calculations determined by Mac to help equalize individual library contributions, based on the results from the MiSeq run:


| Sample  | Volume_4nMsample_toBalance_uL |   |
|---------|-------------------------------|---|
| CH01-06 | 2.43                          |   |
| CH01-14 | 3.1                           |   |
| CH01-22 | 2.58                          |   |
| CH01-38 | 1.63                          |   |
| CH03-04 | 1.66                          |   |
| CH03-15 | 4.03                          |   |
| CH03-33 | 1.71                          |   |
| CH05-01 | 2.1                           |   |
| CH05-06 | 4.38                          |   |
| CH05-21 | 1.84                          |   |
| CH05-24 | 2.98                          |   |
| CH07-06 | 1.79                          |   |
| CH07-11 | 2.83                          |   |
| CH07-24 | 2.98                          |   |
| CH09-02 | 1.36                          |   |
| CH09-13 | 4.57                          |   |
| CH09-28 | 1.4                           |   |
| CH10-01 | 1.29                          |   |
| CH10-08 | 1.83                          |   |
| CH10-11 | 0.99                          |   |


Full Google Sheet for this project:

- [OA Crab Sample Collection 071119](https://docs.google.com/spreadsheets/d/1ym0XnYVts98tIUCn0kIaU6VuvqxzV7LoSx9RHwLdiIs/edit?usp=sharing)
