--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left layout: post title: Samples Submission - Supplemental Ronits C.gigas Diploid-Triploid Ctendidia gDNA for WGBS by ZymoResearch date: '2020-09-01 10:08' tags: - Crassostrea gigas - ctenidia - diploid - triploid - Pacific oyster - WGBS categories: - 2020 - Samples Submitted --- [I re-quantified samples for Zymo whole genome bisulfite sequencing (WGBS) project 3534 earlier today](https://robertslab.github.io/sams-notebook/posts/2020/2020-09-01-DNA-Quantification---Re-quant-Ronits-C.gigas-Diploid-Triploid-Ctenidia-gDNA-Submitted-to-ZymoResearch/) due to ZymoResearch indicating there was insufficient DNA for most of the samples and submitted an additional 1ug of each sample. See table below for volume of DNA sent based on today's (20200901) quants: | Sample_ID | [initial](ng/uL) | [Zymo](ng/uL) | [20200901](ng/uL) | Vol_for_1ug(uL) | |-----------|------------------|---------------|-------------------|-----------------| | D11-C | 181 | 85.20 | 269 | 3.72 | | D12-C | 192 | 46.16 | 289 | 3.46 | | D13-C | 288 | 606.23 | 332 | 3.01 | | D19-C | 63.6 | 11.14 | 51.3 | 19.49 | | D20-C | 123 | 9.40 | 77 | 12.99 | | T11-C | 130 | 41.52 | 88 | 11.36 | | T12-C | 262 | 9.96 | 139 | 7.19 | | T13-C | 250 | 130.14 | 148 | 6.76 | | T19-C | 173 | 35.58 | 92.9 | 10.76 | | T20-C | 109 | 132.10 | 78.4 | 12.76 |