---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
layout: post
title: Sample Submitted - C.gigas Diploid-Triploid pH Treatments Ctenidia to ZymoResearch for WGBS
date: '2020-08-24 09:54'
tags:
  - Crassostrea gigas
  - diploid
  - triploid
  - Pacific oyster
  - ctendidia
  - wgbs
  - BSseq
categories:
  - 2020
  - Miscellaneous
---
Submitted 1.5ug of the [24 _C.gigas_ ctenidia ctenidia gDNA isolated last week](https://robertslab.github.io/sams-notebook/posts/2020/2020-08-21-DNA-Isolation-and-Quantification---C.gigas-High-Low-pH-Triploid-and-Diploid-Ctenidia/) (20200821) to ZymoResearch for whole genome bisulfite sequencing (WGBS) to compare differences in diploid/triploids and responses to elevated pH:

- 150bp PE reads

- ~60M reads/sample


| Sample_ID | Concentration(ng/uL) | Vol_for_1.5ug(uL) |
|-----------|----------------------|-------------------|
| 2N_HI_5   | 40.4                 | 37.1              |
| 2N_HI_8   | 11.6                 | 100.0             |
| 2N_HI_9   | 32.3                 | 46.4              |
| 2N_HI_10  | 61                   | 24.6              |
| 2N_HI_11  | 21                   | 71.4              |
| 2N_HI_12  | 11.2                 | 100.0             |
| 2N_LOW_1  | 32.1                 | 46.7              |
| 2N_LOW_2  | 32.5                 | 46.2              |
| 2N_LOW_3  | 36                   | 41.7              |
| 2N_LOW_4  | 40.2                 | 37.3              |
| 2N_LOW_5  | 17.8                 | 84.3              |
| 2N_LOW_6  | 22.8                 | 65.8              |
| 3N_HI_2   | 29.6                 | 50.7              |
| 3N_HI_3   | 71.8                 | 20.9              |
| 3N_HI_5   | 29.3                 | 51.2              |
| 3N_HI_8   | 38.9                 | 38.6              |
| 3N_HI_10  | 38.3                 | 39.2              |
| 3N_HI_11  | 52.3                 | 28.7              |
| 3N_LOW_6  | 35.3                 | 42.5              |
| 3N_LOW_7  | 43.6                 | 34.4              |
| 3N_LOW_8  | 63.9                 | 23.5              |
| 3N_LOW_10 | 54.4                 | 27.6              |
| 3N_LOW_11 | 50.8                 | 29.5              |
| 3N_LOW_12 | 52                   | 28.8              |

NOTE: Those samples listed as 100.0uL used the entirety of the sample and did not have a full 1.5ug. However, ZymoResearch only needs 1.0ug; sending 1.5ug just ensures they have a bit extra if needed.