---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
layout: post
title: DNA Isolation & Quantification - C.bairdi RNA from Samples 6212_132_9 6212_334_12 6212_485_26
date: '2020-02-11 15:21'
tags:
  - DNA isolation
  - DNA quantification
  - Qubit3.0
  - dsDNA HS assay
  - Tanner crab
categories:
  - 2020
  - Tanner Crab RNAseq
---
Isolated DNA from three samples (see Qubit spreadsheet in "Results" below for sample IDs) using the [Quick DNA/RNA Microprep Kit](https://github.com/RobertsLab/resources/blob/master/protocols/Commercial_Protocols/ZymoResearch_quick-dna-rna_microprep_plus_kit_20190411.pdf) (ZymoResearch; PDF) according to the manufacturer's protocol for liquids/cells in RNAlater.

These samples were from RNA isolations earlier today:

- [20200211](https://robertslab.github.io/sams-notebook/posts/2020/2020-02-11-RNA-Isolation-and-Quantification---C.bairdi-RNA-from-Samples-6212_132_9-6212_334_12-6212_485_26/)


Brief rundown of method:

- Used 35uL from each RNAlater/hemocyte slurry.

- Mixed with equal volume of H<sub>2</sub>O (35uL).

- Retained DNA on the Zymo-Spin IC-XM columns at 4<sup>o</sup>C for isolation after RNA isolation.

- DNA was eluted in 15uL H<sub>2</sub>O

DNA was quantified on the Roberts Lab Qubit 3.0 using the 1x DNA High Sensitivity Assay (Invitrogen), using 1uL of each sample.

---

# RESULTS

Qubit results (Google Sheet):

- [20200211_qubit_crab_gDNA](https://docs.google.com/spreadsheets/d/1PHHUDZNjMyvzRF7IIyzP7F1rMsSLsScqk7KzLaBPRCc/edit?usp=sharing)

Samples were stored at -80<sup>o</sup>C in:

[Rack 15, 4, 5](http://b.link/srlab-80C) in [C.bairdi gDNA Box #2](https://docs.google.com/spreadsheets/d/1EnI5UlvN8qoT3pB0VcP6Eu44BdiyyElToYA9VwukPEE/edit?usp=sharing)


| Sample ID   | [DNA] (ng/uL) |
|-------------|---------------|
| 6212_485_26 | 28.8          |
| 6212_132_9  | 19.7          |
| 6212_334_12 | 23.6          |