---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
layout: post
title: DNA Quality Assessment - Agarose Gel for C.bairdi 20102558-2729 gDNA from 20200122
date: '2020-01-22 14:46'
tags:
  - Tanner crab
  - 20102558-2729
  - DNA isolation
  - Quick DNA/RNA Microprep Kit
  - Qubit 3.0
  - 1x hsDNA Assay
  - Chionoecetes bairdi
categories:
  - 2020
  - Miscellaneous
---
Earlier today, [I isolated gDNA from _C.bairdi_ 20102558-2729 ethanol-preserved muscle tissue using the Quick DNA/RNA MicroPrep Plus Kit (ZymoResearch)](https://robertslab.github.io/sams-notebook/posts/2020/2020-01-22-DNA-Isolation---C.bairdi-20102558-2729-EtOH-preserved-Tissue-via-Three-Variations-Using-Quick-DNA-RNA-MicroPrep-Kit/) and prepared the tissue in three different ways to see how they would compare:

Ran 10uL of each sample on a 0.8% agarose, 1x low TAE gel with ethidium bromide.

Used the GeneRuler DNA Ladder Mix (ThermoFisher) as ladder.


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# RESULTS

Ladder:

![ThermoFisher GeneRuler DNA Ladder Mix](https://github.com/RobertsLab/resources/blob/master/protocols/Commercial_Protocols/ThermoFisher_OgeneRuler_DNA_Ladder_Mix_F100439.jpg?raw=true)

---

From left to right, gel was loaded:

- Ladder

- "damp" sample prep

- "dry" sample prep

- "minced" sample prep

![Gel image](https://github.com/RobertsLab/sams-notebook/blob/master/images/20200122_gel_cbai_quick-DNA.jpg?raw=true)


Well, all three preps look the same - very degraded. This is virtually the same as what I saw using the E.Z.N.A. Mollusc Kit (Omega). Looke like we'll have to pursue a different route to get high quality gDNA for long read sequencing.

Will talk to Grace to see if we have extra hemolymph samples in RNAlater that I can try to get gDNA from and see if that gDNA looks any better.