---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
layout: post
title: Samples Received - Pacific Oyster Tissues from Hawaii (Maria Haws) from High and Low pCO2
date: '2019-12-10 15:04'
tags:
  - tissues
  - mantle
  - gill
  - adductor muscle
  - gonad
  - Maria Haws
  - Daniel Wilkie
  - Crassostrea gigas
  - Pacific oyster
categories:
  - 2019
  - Samples Received
---
We received frozen _Crassostrea gigas_ tissues from Maria Haws in Hawaii, as part of a pCO<sub>2</sub> experiment. Tissues include:

- adductor muscle (A)

- gill (G)

- gonad (N)

- mantle (M)

Tubes were boxed and stored in the -80<sup>o</sup>C:

- [Rack 3, Column 4, Rows 1 & 3](https://docs.google.com/spreadsheets/d/1Qsvz3QTURlPF_hX05BQxjom3484WuMfqQ1ILl9LEljU/edit#gid=2006985773)



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![two bags of frozen tissues, low and high pH](https://github.com/RobertsLab/sams-notebook/blob/master/images/20191210_samples_cgigas_tissues_hawaii_high-low_pC02-01.jpg?raw=true)

---

![high pH samples removed from bag](https://github.com/RobertsLab/sams-notebook/blob/master/images/20191210_samples_cgigas_tissues_hawaii_high-low_pC02-02.jpg?raw=true)

---

![low pH samples removed from bag](https://github.com/RobertsLab/sams-notebook/blob/master/images/20191210_samples_cgigas_tissues_hawaii_high-low_pC02-03.jpg?raw=true)


---

The low pH samples had two casualties:

- Sample '1LA' has no tube; just a lid

- Sample '3LN' lid popped up in transit and tissue is missing

![low pH samples in freezer box](https://github.com/RobertsLab/sams-notebook/blob/master/images/20191210_samples_cgigas_tissues_hawaii_high-low_pC02-04.jpg?raw=true)

---

![high pH samples in freezer box](https://github.com/RobertsLab/sams-notebook/blob/master/images/20191210_samples_cgigas_tissues_hawaii_high-low_pC02-05.jpg?raw=true)